1k5k
From Proteopedia
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[[Image:1k5k.gif|left|200px]] | [[Image:1k5k.gif|left|200px]] | ||
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'''Homonuclear 1H Nuclear Magnetic Resonance Assignment and Structural Characterization of HIV-1 Tat Mal Protein''' | '''Homonuclear 1H Nuclear Magnetic Resonance Assignment and Structural Characterization of HIV-1 Tat Mal Protein''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1K5K is a [[Single protein]] structure | + | 1K5K is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K5K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Peloponese, J M.]] | [[Category: Peloponese, J M.]] | ||
[[Category: Solomiac, M.]] | [[Category: Solomiac, M.]] | ||
| - | [[Category: | + | [[Category: African variant]] |
| - | [[Category: | + | [[Category: Hiv-1]] |
| - | [[Category: | + | [[Category: Nmr.]] |
| - | [[Category: | + | [[Category: Regulatory protein]] |
| - | [[Category: | + | [[Category: Tat]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:20:18 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 19:20, 2 May 2008
Homonuclear 1H Nuclear Magnetic Resonance Assignment and Structural Characterization of HIV-1 Tat Mal Protein
Overview
The transacting transcriptional activator (Tat) is a viral protein essential for activation of the human immunodeficiency virus (HIV) genes, and it plays an important role in HIV induced immunodeficiency. We report the NMR structural characterization of the active Tat Mal variant that belongs to a highly virulent D-subtype HIV type-1 (HIV-1) strain (Mal) found mainly in Africa. A full Tat Mal protein (87 residues) is synthesized. This synthetic protein is active in a transactivation assay with HeLa cells infected with the HIV long terminal repeated noncoding sequences of the HIV-1 provirus (LTR) lac Z gene. Homonuclear (1)H-NMR spectra allows the sequential assignment of the Tat Mal spin systems. Simulating annealing generates 20 conformers with similar folding. The geometry of the mean structure is optimized with energy minimization to obtain a final structure. As the European variant (Tat Bru) the N-terminal region of Tat Mal constitutes the core, and there is a hydrophobic pocket composed of the conserved Trp 11 interacting with several aromatic residues. The two functional regions of Tat (basic and the cysteine-rich regions) are well exposed to the solvent. A short alpha-helix is observed in region V adjacent to the basic region. This alpha helix induces local structural variations compared to the NMR structure of Tat Bru, and it brings the cysteine-rich and basic regions closer. This study suggests that similar folding exists among Tat variants.
About this Structure
1K5K is a Single protein structure. Full crystallographic information is available from OCA.
Reference
Homonuclear (1)H-NMR assignment and structural characterization of human immunodeficiency virus type 1 Tat Mal protein., Gregoire C, Peloponese JM Jr, Esquieu D, Opi S, Campbell G, Solomiac M, Lebrun E, Lebreton J, Loret EP, Biopolymers. 2001;62(6):324-35. PMID:11857271 Page seeded by OCA on Fri May 2 22:20:18 2008
Categories: Single protein | Campbell, G. | Esquieu, D. | Gregoire, C. | Lebreton, J. | Lebrun, E. | Loret, E P. | Opi, S. | Peloponese, J M. | Solomiac, M. | African variant | Hiv-1 | Nmr. | Regulatory protein | Tat
