1kfv

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[[Image:1kfv.gif|left|200px]]
[[Image:1kfv.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1kfv |SIZE=350|CAPTION= <scene name='initialview01'>1kfv</scene>, resolution 2.55&Aring;
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The line below this paragraph, containing "STRUCTURE_1kfv", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PDI:PHOSPHORIC+ACID+MONO-(3-HYDROXY-PROPYL)+ESTER'>PDI</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-formamidopyrimidine_glycosylase DNA-formamidopyrimidine glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.23 3.2.2.23] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= MUTM or FPG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1358 Lactococcus lactis])
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-->
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|DOMAIN=
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{{STRUCTURE_1kfv| PDB=1kfv | SCENE= }}
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|RELATEDENTRY=[[1ee8|1EE8]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kfv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kfv OCA], [http://www.ebi.ac.uk/pdbsum/1kfv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kfv RCSB]</span>
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}}
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'''Crystal Structure of Lactococcus lactis Formamido-pyrimidine DNA Glycosylase (alias Fpg or MutM) Non Covalently Bound to an AP Site Containing DNA.'''
'''Crystal Structure of Lactococcus lactis Formamido-pyrimidine DNA Glycosylase (alias Fpg or MutM) Non Covalently Bound to an AP Site Containing DNA.'''
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[[Category: Serre, L.]]
[[Category: Serre, L.]]
[[Category: Zelwer, C.]]
[[Category: Zelwer, C.]]
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[[Category: abasic site]]
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[[Category: Abasic site]]
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[[Category: ap lyase]]
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[[Category: Ap lyase]]
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[[Category: dna]]
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[[Category: Dna]]
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[[Category: dna repair enzyme]]
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[[Category: Dna repair enzyme]]
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[[Category: n-glycosylase]]
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[[Category: N-glycosylase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:41:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:48:02 2008''
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Revision as of 19:41, 2 May 2008

Image:1kfv.gif

Template:STRUCTURE 1kfv

Crystal Structure of Lactococcus lactis Formamido-pyrimidine DNA Glycosylase (alias Fpg or MutM) Non Covalently Bound to an AP Site Containing DNA.


Overview

The formamidopyrimidine-DNA glycosylase (Fpg, MutM) is a bifunctional base excision repair enzyme (DNA glycosylase/AP lyase) that removes a wide range of oxidized purines, such as 8-oxoguanine and imidazole ring-opened purines, from oxidatively damaged DNA. The structure of a non-covalent complex between the Lactoccocus lactis Fpg and a 1,3-propanediol (Pr) abasic site analogue-containing DNA has been solved. Through an asymmetric interaction along the damaged strand and the intercalation of the triad (M75/R109/F111), Fpg pushes out the Pr site from the DNA double helix, recognizing the cytosine opposite the lesion and inducing a 60 degrees bend of the DNA. The specific recognition of this cytosine provides some structural basis for understanding the divergence between Fpg and its structural homologue endo nuclease VIII towards their substrate specificities. In addition, the modelling of the 8-oxoguanine residue allows us to define an enzyme pocket that may accommodate the extrahelical oxidized base.

About this Structure

1KFV is a Single protein structure of sequence from Lactococcus lactis. Full crystallographic information is available from OCA.

Reference

Crystal structure of the Lactococcus lactis formamidopyrimidine-DNA glycosylase bound to an abasic site analogue-containing DNA., Serre L, Pereira de Jesus K, Boiteux S, Zelwer C, Castaing B, EMBO J. 2002 Jun 17;21(12):2854-65. PMID:12065399 Page seeded by OCA on Fri May 2 22:41:44 2008

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