1khc
From Proteopedia
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'''Crystal Structure of the PWWP Domain of Mammalian DNA Methyltransferase Dnmt3b''' | '''Crystal Structure of the PWWP Domain of Mammalian DNA Methyltransferase Dnmt3b''' | ||
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==Reference== | ==Reference== | ||
The PWWP domain of mammalian DNA methyltransferase Dnmt3b defines a new family of DNA-binding folds., Qiu C, Sawada K, Zhang X, Cheng X, Nat Struct Biol. 2002 Mar;9(3):217-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11836534 11836534] | The PWWP domain of mammalian DNA methyltransferase Dnmt3b defines a new family of DNA-binding folds., Qiu C, Sawada K, Zhang X, Cheng X, Nat Struct Biol. 2002 Mar;9(3):217-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11836534 11836534] | ||
| - | [[Category: DNA (cytosine-5-)-methyltransferase]] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Sawada, K.]] | [[Category: Sawada, K.]] | ||
[[Category: Zhang, X.]] | [[Category: Zhang, X.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:44:52 2008'' | |
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Revision as of 19:44, 2 May 2008
Crystal Structure of the PWWP Domain of Mammalian DNA Methyltransferase Dnmt3b
Overview
The PWWP domain is a weakly conserved sequence motif found in > 60 eukaryotic proteins, including the mammalian DNA methyltransferases Dnmt3a and Dnmt3b. These proteins often contain other chromatin-association domains. A 135-residue PWWP domain from mouse Dnmt3b (amino acids 223--357) has been structurally characterized at 1.8 A resolution. The N-terminal half of this domain resembles a barrel-like five-stranded structure, whereas the C-terminal half contains a five-helix bundle. The two halves are packed against each other to form a single structural module that exhibits a prominent positive electrostatic potential. The PWWP domain alone binds DNA in vitro, probably through its basic surface. We also show that recombinant Dnmt3b2 protein (a splice variant of Dnmt3b) and two N-terminal deletion mutants (Delta218 and Delta369) have approximately equal methyl transfer activity on unmethylated and hemimethylated CpG-containing oligonucleotides. The Delta218 protein, which includes the PWWP domain, binds DNA more strongly than Delta369, which lacks the PWWP domain.
About this Structure
1KHC is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
The PWWP domain of mammalian DNA methyltransferase Dnmt3b defines a new family of DNA-binding folds., Qiu C, Sawada K, Zhang X, Cheng X, Nat Struct Biol. 2002 Mar;9(3):217-24. PMID:11836534 Page seeded by OCA on Fri May 2 22:44:52 2008
