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7ta8
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7ta8 is ON HOLD Authors: Description: Category: Unreleased Structures) |
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| - | '''Unreleased structure''' | ||
| - | + | ==NMR structure of crosslinked cyclophilin A== | |
| + | <StructureSection load='7ta8' size='340' side='right'caption='[[7ta8]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7ta8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TA8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ta8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ta8 OCA], [https://pdbe.org/7ta8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ta8 RCSB], [https://www.ebi.ac.uk/pdbsum/7ta8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ta8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PPIA_HUMAN PPIA_HUMAN] PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Fluorosubstituted tryptophans serve as valuable probes for fluorescence and nuclear magnetic resonance (NMR) studies of proteins. Here, we describe an unusual photoreactivity introduced by replacing the single tryptophan in cyclophilin A with 7-fluoro-tryptophan. UV exposure at 282 nm defluorinates 7-fluoro-tryptophan and crosslinks it to a nearby phenylalanine, generating a bright fluorophore. The crosslink-containing fluorescent protein possesses a large quantum yield of approximately 0.40 with a fluorescence lifetime of 2.38 ns. The chemical nature of the crosslink and the three-dimensional protein structure were determined by mass spectrometry and NMR spectroscopy. To the best of our knowledge, this is the first report of a Phe-Trp crosslink in a protein. Our finding may break new ground for developing novel fluorescence probes and for devising new strategies to exploit aromatic crosslinks in proteins. | ||
| - | + | The Magic of Linking Rings: Discovery of a Unique Photoinduced Fluorescent Protein Crosslink.,Lu M, Toptygin D, Xiang Y, Shi Y, Schwieters CD, Lipinski EC, Ahn J, Byeon IL, Gronenborn AM J Am Chem Soc. 2022 Jun 22;144(24):10809-10816. doi: 10.1021/jacs.2c02054. Epub, 2022 May 14. PMID:35574633<ref>PMID:35574633</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7ta8" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Cyclophilin 3D structures|Cyclophilin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Ahn J]] | ||
| + | [[Category: Byeon I-JL]] | ||
| + | [[Category: Gronenborn AM]] | ||
| + | [[Category: Lipinski EC]] | ||
| + | [[Category: Lu M]] | ||
| + | [[Category: Schwieters CD]] | ||
| + | [[Category: Shi Y]] | ||
| + | [[Category: Toptygin D]] | ||
| + | [[Category: Xiang Y]] | ||
Current revision
NMR structure of crosslinked cyclophilin A
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Categories: Homo sapiens | Large Structures | Ahn J | Byeon I-JL | Gronenborn AM | Lipinski EC | Lu M | Schwieters CD | Shi Y | Toptygin D | Xiang Y
