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2ruj
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution structure of MTSL spin-labeled Schizosaccharomyces pombe Sin1 CRIM domain== | |
| + | <StructureSection load='2ruj' size='340' side='right'caption='[[2ruj]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2ruj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RUJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RUJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=R1A:3-{[(2,2,5,5-TETRAMETHYL-1-OXO-2,5-DIHYDRO-1H-PYRROLIUM-3-YL)METHYL]DISULFANYL}-D-ALANINE'>R1A</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ruj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ruj OCA], [https://pdbe.org/2ruj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ruj RCSB], [https://www.ebi.ac.uk/pdbsum/2ruj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ruj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SIN1_SCHPO SIN1_SCHPO] Interacts with the sty1 MAP kinase and has a role in the timing of the initiation of mitosis.<ref>PMID:10428959</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | NMR structure determination of soluble proteins depends in large part on distance restraints derived from NOE. In this study, we examined the impact of paramagnetic relaxation enhancement (PRE)-derived distance restraints on protein structure determination. A high-resolution structure of the loop-rich soluble protein Sin1 could not be determined by conventional NOE-based procedures due to an insufficient number of NOE restraints. By using the 867 PRE-derived distance restraints obtained from the NOE-based structure determination procedure, a high-resolution structure of Sin1 could be successfully determined. The convergence and accuracy of the determined structure were improved by increasing the number of PRE-derived distance restraints. This study demonstrates that PRE-derived distance restraints are useful in the determination of a high-resolution structure of a soluble protein when the number of NOE constraints is insufficient. | ||
| - | + | Utilization of paramagnetic relaxation enhancements for high-resolution NMR structure determination of a soluble loop-rich protein with sparse NOE distance restraints.,Furuita K, Kataoka S, Sugiki T, Hattori Y, Kobayashi N, Ikegami T, Shiozaki K, Fujiwara T, Kojima C J Biomol NMR. 2015 Jan;61(1):55-64. doi: 10.1007/s10858-014-9882-7. Epub 2014 Nov, 27. PMID:25428765<ref>PMID:25428765</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 2ruj" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Furuita | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| - | [[Category: | + | [[Category: Fujiwara T]] |
| + | [[Category: Furuita K]] | ||
| + | [[Category: Ikegami T]] | ||
| + | [[Category: Kataoka S]] | ||
| + | [[Category: Kobayashi N]] | ||
| + | [[Category: Kojima C]] | ||
| + | [[Category: Shiozaki K]] | ||
| + | [[Category: Sugiki T]] | ||
Current revision
Solution structure of MTSL spin-labeled Schizosaccharomyces pombe Sin1 CRIM domain
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