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| | <StructureSection load='4xo1' size='340' side='right'caption='[[4xo1]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='4xo1' size='340' side='right'caption='[[4xo1]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xo1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XO1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XO1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xo1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XO1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XO1 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xo2|4xo2]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xo1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xo1 OCA], [https://pdbe.org/4xo1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xo1 RCSB], [https://www.ebi.ac.uk/pdbsum/4xo1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xo1 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gnsA, yccL, b4517, JW0976 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xo1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xo1 OCA], [http://pdbe.org/4xo1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xo1 RCSB], [http://www.ebi.ac.uk/pdbsum/4xo1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xo1 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GNSA_ECOLI GNSA_ECOLI]] Overexpression increases levels of unsaturated fatty acids and suppresses both the temperature-sensitive fabA6 mutation and cold-sensitive secG null mutation.<ref>PMID:11544213</ref> | + | [https://www.uniprot.org/uniprot/GNSA_ECOLI GNSA_ECOLI] Overexpression increases levels of unsaturated fatty acids and suppresses both the temperature-sensitive fabA6 mutation and cold-sensitive secG null mutation.<ref>PMID:11544213</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Dong, Y]] | + | [[Category: Dong Y]] |
| - | [[Category: Gao, Z]] | + | [[Category: Gao Z]] |
| - | [[Category: Zhan, L]] | + | [[Category: Zhan L]] |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Suppressor]]
| + | |
| Structural highlights
Function
GNSA_ECOLI Overexpression increases levels of unsaturated fatty acids and suppresses both the temperature-sensitive fabA6 mutation and cold-sensitive secG null mutation.[1]
Publication Abstract from PubMed
Escherichia Coli GnsA is a regulator of phosphatidylethanolamine synthesis and functions as a suppressor of both a secG null mutation and fabA6 mutations. GnsA may also be a toxin with the cognate antitoxin YmcE. Here we report the crystal structure of GnsA to 1.8 A. GnsA forms a V shaped hairpin structure that is tightly associated into a homodimer. Our comprehensive structural study suggests that GnsA is structurally similar to an outer membrane protein, suggesting a function of protein binding.
Crystal structure of GnsA from Escherichia coli.,Wei Y, Zhan L, Gao Z, Prive GG, Dong Y Biochem Biophys Res Commun. 2015 Jun 19;462(1):1-7. doi:, 10.1016/j.bbrc.2015.03.133. Epub 2015 Apr 1. PMID:25839658[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sugai R, Shimizu H, Nishiyama K, Tokuda H. Overexpression of yccL (gnsA) and ydfY (gnsB) increases levels of unsaturated fatty acids and suppresses both the temperature-sensitive fabA6 mutation and cold-sensitive secG null mutation of Escherichia coli. J Bacteriol. 2001 Oct;183(19):5523-8. PMID:11544213 doi:http://dx.doi.org/10.1128/JB.183.19.5523-5528.2001
- ↑ Wei Y, Zhan L, Gao Z, Prive GG, Dong Y. Crystal structure of GnsA from Escherichia coli. Biochem Biophys Res Commun. 2015 Jun 19;462(1):1-7. doi:, 10.1016/j.bbrc.2015.03.133. Epub 2015 Apr 1. PMID:25839658 doi:http://dx.doi.org/10.1016/j.bbrc.2015.03.133
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