|
|
| Line 3: |
Line 3: |
| | <StructureSection load='4xrw' size='340' side='right'caption='[[4xrw]], [[Resolution|resolution]] 1.79Å' scene=''> | | <StructureSection load='4xrw' size='340' side='right'caption='[[4xrw]], [[Resolution|resolution]] 1.79Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4xrw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_orientalis"_pittenger_and_brigham_1956 "streptomyces orientalis" pittenger and brigham 1956]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XRW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XRW FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4xrw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Amycolatopsis_orientalis Amycolatopsis orientalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XRW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XRW FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3tfz|3tfz]], [[2rer|2rer]], [[2rez|2rez]], [[2kf2|2kf2]], [[4xrt|4xrt]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xrw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xrw OCA], [https://pdbe.org/4xrw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xrw RCSB], [https://www.ebi.ac.uk/pdbsum/4xrw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xrw ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bexL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=31958 "Streptomyces orientalis" Pittenger and Brigham 1956])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xrw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xrw OCA], [http://pdbe.org/4xrw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xrw RCSB], [http://www.ebi.ac.uk/pdbsum/4xrw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xrw ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D7RFJ9_AMYOR D7RFJ9_AMYOR] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Streptomyces orientalis pittenger and brigham 1956]] | + | [[Category: Amycolatopsis orientalis]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Aguilar, S]] | + | [[Category: Aguilar S]] |
| - | [[Category: Barajas, J F]] | + | [[Category: Barajas JF]] |
| - | [[Category: Caldara-Festin, G M]] | + | [[Category: Caldara-Festin GM]] |
| - | [[Category: Jackson, D R]] | + | [[Category: Jackson DR]] |
| - | [[Category: Khanna, A]] | + | [[Category: Khanna A]] |
| - | [[Category: Liu, H W]] | + | [[Category: Liu H-W]] |
| - | [[Category: Nguyen, M]] | + | [[Category: Nguyen M]] |
| - | [[Category: Patel, A]] | + | [[Category: Patel A]] |
| - | [[Category: Sasaki, E]] | + | [[Category: Sasaki E]] |
| - | [[Category: Tsai, S C]] | + | [[Category: Tsai SC]] |
| - | [[Category: Valentic, T R]] | + | [[Category: Valentic TR]] |
| - | [[Category: Vo, M]] | + | [[Category: Vo M]] |
| - | [[Category: Aro/cyc]]
| + | |
| - | [[Category: Aromatase/cyclase]]
| + | |
| - | [[Category: Dehydratase]]
| + | |
| - | [[Category: Helix-grip fold]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | [[Category: Natural product]]
| + | |
| - | [[Category: Polyketide]]
| + | |
| - | [[Category: Polyketide synthase]]
| + | |
| Structural highlights
Function
D7RFJ9_AMYOR
Publication Abstract from PubMed
Aromatic polyketides make up a large class of natural products with diverse bioactivity. During biosynthesis, linear poly-beta-ketone intermediates are regiospecifically cyclized, yielding molecules with defined cyclization patterns that are crucial for polyketide bioactivity. The aromatase/cyclases (ARO/CYCs) are responsible for regiospecific cyclization of bacterial polyketides. The two most common cyclization patterns are C7-C12 and C9-C14 cyclizations. We have previously characterized three monodomain ARO/CYCs: ZhuI, TcmN, and WhiE. The last remaining uncharacterized class of ARO/CYCs is the di-domain ARO/CYCs, which catalyze C7-C12 cyclization and/or aromatization. Di-domain ARO/CYCs can further be separated into two subclasses: "nonreducing" ARO/CYCs, which act on nonreduced poly-beta-ketones, and "reducing" ARO/CYCs, which act on cyclized C9 reduced poly-beta-ketones. For years, the functional role of each domain in cyclization and aromatization for di-domain ARO/CYCs has remained a mystery. Here we present what is to our knowledge the first structural and functional analysis, along with an in-depth comparison, of the nonreducing (StfQ) and reducing (BexL) di-domain ARO/CYCs. This work completes the structural and functional characterization of mono- and di-domain ARO/CYCs in bacterial type II polyketide synthases and lays the groundwork for engineered biosynthesis of new bioactive polyketides.
Structural and functional analysis of two di-domain aromatase/cyclases from type II polyketide synthases.,Caldara-Festin G, Jackson DR, Barajas JF, Valentic TR, Patel AB, Aguilar S, Nguyen M, Vo M, Khanna A, Sasaki E, Liu HW, Tsai SC Proc Natl Acad Sci U S A. 2015 Dec 2. pii: 201512976. PMID:26631750[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Caldara-Festin G, Jackson DR, Barajas JF, Valentic TR, Patel AB, Aguilar S, Nguyen M, Vo M, Khanna A, Sasaki E, Liu HW, Tsai SC. Structural and functional analysis of two di-domain aromatase/cyclases from type II polyketide synthases. Proc Natl Acad Sci U S A. 2015 Dec 2. pii: 201512976. PMID:26631750 doi:http://dx.doi.org/10.1073/pnas.1512976112
|
