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| | <StructureSection load='4yh8' size='340' side='right'caption='[[4yh8]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='4yh8' size='340' side='right'caption='[[4yh8]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4yh8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YH8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YH8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4yh8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YH8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPAP8A3.06 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), prp2, mis11, SPBC146.07 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yh8 OCA], [https://pdbe.org/4yh8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yh8 RCSB], [https://www.ebi.ac.uk/pdbsum/4yh8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yh8 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4yh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yh8 OCA], [http://pdbe.org/4yh8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4yh8 RCSB], [http://www.ebi.ac.uk/pdbsum/4yh8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4yh8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/U2AF1_SCHPO U2AF1_SCHPO]] Necessary for the splicing of pre-mRNA. The SF1-U2AF59-U2AF23 complex has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.<ref>PMID:12374752</ref> [[http://www.uniprot.org/uniprot/U2AF2_SCHPO U2AF2_SCHPO]] Necessary for the splicing of pre-mRNA. The SF1-U2AF59-U2AF23 complex has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.<ref>PMID:12374752</ref> | + | [https://www.uniprot.org/uniprot/U2AF1_SCHPO U2AF1_SCHPO] Necessary for the splicing of pre-mRNA. The SF1-U2AF59-U2AF23 complex has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.<ref>PMID:12374752</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fission yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Obayashi, E]] | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| - | [[Category: Park, S Y]] | + | [[Category: Obayashi E]] |
| - | [[Category: Urano, T]] | + | [[Category: Park SY]] |
| - | [[Category: Yoshida, H]] | + | [[Category: Urano T]] |
| - | [[Category: 3' splice site]]
| + | [[Category: Yoshida H]] |
| - | [[Category: Rrm]]
| + | |
| - | [[Category: Splicing]]
| + | |
| - | [[Category: Zn-finger]]
| + | |
| Structural highlights
Function
U2AF1_SCHPO Necessary for the splicing of pre-mRNA. The SF1-U2AF59-U2AF23 complex has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.[1]
Publication Abstract from PubMed
The pre-mRNA splicing reaction of eukaryotic cells has to be carried out extremely accurately, as failure to recognize the splice sites correctly causes serious disease. The small subunit of the U2AF heterodimer is essential for the determination of 3' splice sites in pre-mRNA splicing, and several single-residue mutations of the U2AF small subunit cause severe disorders such as myelodysplastic syndromes. However, the mechanism of RNA recognition is poorly understood. Here we solved the crystal structure of the U2AF small subunit (U2AF23) from fission yeast, consisting of an RNA recognition motif (RRM) domain flanked by two conserved CCCH-type zinc fingers (ZFs). The two ZFs are positioned side by side on the beta sheet of the RRM domain. Further mutational analysis revealed that the ZFs bind cooperatively to the target RNA sequence, but the RRM domain acts simply as a scaffold to organize the ZFs and does not itself contact the RNA directly. This completely novel and unexpected mode of RNA-binding mechanism by the U2AF small subunit sheds light on splicing errors caused by mutations of this highly conserved protein.
A novel 3' splice site recognition by the two zinc fingers in the U2AF small subunit.,Yoshida H, Park SY, Oda T, Akiyoshi T, Sato M, Shirouzu M, Tsuda K, Kuwasako K, Unzai S, Muto Y, Urano T, Obayashi E Genes Dev. 2015 Aug 1;29(15):1649-60. doi: 10.1101/gad.267104.115. Epub 2015 Jul , 27. PMID:26215567[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Huang T, Vilardell J, Query CC. Pre-spliceosome formation in S.pombe requires a stable complex of SF1-U2AF(59)-U2AF(23). EMBO J. 2002 Oct 15;21(20):5516-26. PMID:12374752
- ↑ Yoshida H, Park SY, Oda T, Akiyoshi T, Sato M, Shirouzu M, Tsuda K, Kuwasako K, Unzai S, Muto Y, Urano T, Obayashi E. A novel 3' splice site recognition by the two zinc fingers in the U2AF small subunit. Genes Dev. 2015 Aug 1;29(15):1649-60. doi: 10.1101/gad.267104.115. Epub 2015 Jul , 27. PMID:26215567 doi:http://dx.doi.org/10.1101/gad.267104.115
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