This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
4yn7
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4yn7 is ON HOLD Authors: Yang, X., Jiang, T. Description: Category: Unreleased Structures Category: Yang, X Category: Jiang, T) |
|||
| (4 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Non-oxidized YfiR== | |
| + | <StructureSection load='4yn7' size='340' side='right'caption='[[4yn7]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4yn7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YN7 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yn7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yn7 OCA], [https://pdbe.org/4yn7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yn7 RCSB], [https://www.ebi.ac.uk/pdbsum/4yn7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yn7 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9I4L4_PSEAE Q9I4L4_PSEAE] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | YfiBNR is a recently identified c-di-GMP regulatory system involved in bacterial biofilm formation. The periplasmic protein YfiR inhibits the diguanylate cyclase activity of the inner membrane protein YfiN, whereas YfiB in the outer membrane can release this inhibition by sequestration of YfiR. In addition, this system may respond to anoxic conditions via YfiR, although the detailed mechanism is still unknown. Here we report crystal structures of Pseudomonas aeruginosa YfiR in the absence and presence of oxidative glutathione. Our structures reveal the overall folding of YfiR for the first time and demonstrate that YfiR exist as a dimer. Comparison of the two structures in different redox states revealed a broken/formation of one disulfide bond (Cys71-Cys110) and local conformational change around the other one (Cys145-Cys152). Mutagenesis studies indicated that Cys145-Cys152 plays an important role in maintaining the correct folding of YfiR. | ||
| - | + | Crystal structures of YfiR from Pseudomonas aeruginosa in two redox states.,Yang X, Yang XA, Xu M, Zhou L, Fan Z, Jiang T Biochem Biophys Res Commun. 2015 Apr 4. pii: S0006-291X(15)00628-2. doi:, 10.1016/j.bbrc.2015.03.160. PMID:25849887<ref>PMID:25849887</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 4yn7" style="background-color:#fffaf0;"></div> |
| - | [[Category: Jiang | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas aeruginosa PAO1]] | ||
| + | [[Category: Jiang T]] | ||
| + | [[Category: Yang X]] | ||
Current revision
Non-oxidized YfiR
| |||||||||||
