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| | ==Crystal structure of the extracellular receptor domain of the essential sensor kinase WalK from Staphylococcus aureus== | | ==Crystal structure of the extracellular receptor domain of the essential sensor kinase WalK from Staphylococcus aureus== |
| - | <StructureSection load='4ywz' size='340' side='right' caption='[[4ywz]], [[Resolution|resolution]] 1.70Å' scene=''> | + | <StructureSection load='4ywz' size='340' side='right'caption='[[4ywz]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ywz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YWZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YWZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ywz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YWZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YWZ FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ywz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ywz OCA], [https://pdbe.org/4ywz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ywz RCSB], [https://www.ebi.ac.uk/pdbsum/4ywz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ywz ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ywz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ywz OCA], [http://pdbe.org/4ywz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ywz RCSB], [http://www.ebi.ac.uk/pdbsum/4ywz PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/WALK_STAAB WALK_STAAB]] Member of the two-component regulatory system WalK/WalR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to WalR (By similarity). | + | [https://www.uniprot.org/uniprot/WALK_STAAE WALK_STAAE] Member of the two-component regulatory system WalK/WalR that regulates genes involved in cell wall metabolism, virulence regulation, biofilm production, oxidative stress resistance and antibiotic resistance via direct or indirect regulation of autolysins. Functions as a sensor protein kinase which is autophosphorylated at a histidine residue in the dimerization domain and transfers its phosphate group to the conserved aspartic acid residue in the regulatory domain of WalR. In turn, WalR binds to the upstream promoter regions of the target genes to positively and negatively regulate their expression.[UniProtKB:Q9RDT3] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Histidine kinase]] | + | [[Category: Large Structures]] |
| - | [[Category: He, C]]
| + | |
| - | [[Category: Ji, Q]]
| + | |
| - | [[Category: Wawrzak, Z]]
| + | |
| - | [[Category: Essential]]
| + | |
| - | [[Category: Extracellular receptor]]
| + | |
| - | [[Category: Kinase]]
| + | |
| | [[Category: Staphylococcus aureus]] | | [[Category: Staphylococcus aureus]] |
| - | [[Category: Transferase]] | + | [[Category: He C]] |
| | + | [[Category: Ji Q]] |
| | + | [[Category: Wawrzak Z]] |
| Structural highlights
Function
WALK_STAAE Member of the two-component regulatory system WalK/WalR that regulates genes involved in cell wall metabolism, virulence regulation, biofilm production, oxidative stress resistance and antibiotic resistance via direct or indirect regulation of autolysins. Functions as a sensor protein kinase which is autophosphorylated at a histidine residue in the dimerization domain and transfers its phosphate group to the conserved aspartic acid residue in the regulatory domain of WalR. In turn, WalR binds to the upstream promoter regions of the target genes to positively and negatively regulate their expression.[UniProtKB:Q9RDT3]
Publication Abstract from PubMed
Most low GC Gram-positive bacteria possess an essential walKR two-component system (TCS) for signal transduction involved in regulating cell wall homoeostasis. Despite the well-established intracellular regulatory mechanism, the role of this TCS in extracellular signal recognition and factors that modulate the activity of this TCS remain largely unknown. Here we identify the extracellular receptor of the kinase 'WalK' (erWalK) as a key hub for bridging extracellular signal input and intracellular kinase activity modulation in Staphylococcus aureus. Characterization of the crystal structure of erWalK revealed a canonical Per-Arnt-Sim (PAS) domain for signal sensing. Single amino-acid mutation of potential signal-transduction residues resulted in severely impaired function of WalKR. A small molecule derived from structure-based virtual screening against erWalK is capable of selectively activating the walKR TCS. The molecular level characterization of erWalK will not only facilitate exploration of natural signal(s) but also provide a template for rational design of erWalK inhibitors.
Structure and mechanism of the essential two-component signal-transduction system WalKR in Staphylococcus aureus.,Ji Q, Chen PJ, Qin G, Deng X, Hao Z, Wawrzak Z, Yeo WS, Quang JW, Cho H, Luo GZ, Weng X, You Q, Luan CH, Yang X, Bae T, Yu K, Jiang H, He C Nat Commun. 2016 Mar 18;7:11000. doi: 10.1038/ncomms11000. PMID:26987594[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ji Q, Chen PJ, Qin G, Deng X, Hao Z, Wawrzak Z, Yeo WS, Quang JW, Cho H, Luo GZ, Weng X, You Q, Luan CH, Yang X, Bae T, Yu K, Jiang H, He C. Structure and mechanism of the essential two-component signal-transduction system WalKR in Staphylococcus aureus. Nat Commun. 2016 Mar 18;7:11000. doi: 10.1038/ncomms11000. PMID:26987594 doi:http://dx.doi.org/10.1038/ncomms11000
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