This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4z7k

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 07:42, 10 May 2023

Crystal structure of CRISPR RNA processing endoribonuclease Cas6b

Structural highlights

4z7k is a 3 chain structure with sequence from Methanococcus maripaludis and Methanococcus maripaludis C5. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A4FXZ3_METM5

Publication Abstract from PubMed

A subclass of recently discovered CRISPR repeat RNA in bacteria contains minimally recognizable structural features that facilitate an unknown mechanism of recognition and processing by the Cas6 family of endoribonucleases. Cocrystal structures of Cas6 from Methanococcus maripaludis (MmCas6b) bound with its repeat RNA revealed a dual site binding structure and a cleavage site conformation poised for phosphodiester bond breakage. Two non-interacting MmCas6b bind to two separate AAYAA motifs within the same repeat, one distal and one adjacent to the cleavage site. This bound structure potentially competes with a stable but non-productive RNA structure. At the cleavage site, MmCas6b supplies a base pair mimic to stabilize a short 2 base pair stem immediately upstream of the scissile phosphate. Complementary biochemical analyses support the dual-AAYAA binding model and a critical role of the protein-RNA base pair mimic. Our results reveal a previously unknown method of processing non-stem-loop CRISPR RNA by Cas6.

A Non-Stem-Loop CRISPR RNA Is Processed by Dual Binding Cas6.,Shao Y, Richter H, Sun S, Sharma K, Urlaub H, Randau L, Li H Structure. 2016 Apr 5;24(4):547-554. doi: 10.1016/j.str.2016.02.009. Epub 2016, Mar 17. PMID:26996962^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shao Y, Richter H, Sun S, Sharma K, Urlaub H, Randau L, Li H. A Non-Stem-Loop CRISPR RNA Is Processed by Dual Binding Cas6. Structure. 2016 Apr 5;24(4):547-554. doi: 10.1016/j.str.2016.02.009. Epub 2016, Mar 17. PMID:26996962 doi:http://dx.doi.org/10.1016/j.str.2016.02.009

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4z7k"

Categories: Large Structures | Methanococcus maripaludis | Methanococcus maripaludis C5 | Li H

@@ Line 3: / Line 3: @@
 <StructureSection load='4z7k' size='340' side='right'caption='[[4z7k]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4z7k]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Metm5 Metm5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z7K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z7K FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4z7k]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanococcus_maripaludis Methanococcus maripaludis] and [https://en.wikipedia.org/wiki/Methanococcus_maripaludis_C5 Methanococcus maripaludis C5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z7K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z7K FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4z7l|4z7l]]</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z7k OCA], [https://pdbe.org/4z7k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z7k RCSB], [https://www.ebi.ac.uk/pdbsum/4z7k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z7k ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MmarC5_0767 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=402880 METM5])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z7k OCA], [http://pdbe.org/4z7k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z7k RCSB], [http://www.ebi.ac.uk/pdbsum/4z7k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z7k ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A4FXZ3_METM5 A4FXZ3_METM5]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 25: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Metm5]]
+[[Category: Methanococcus maripaludis]]
-[[Category: Li, H]]
+[[Category: Methanococcus maripaludis C5]]
-[[Category: Cas6]]
+[[Category: Li H]]
-[[Category: Crispr rna]]
-[[Category: Endoribonuclease]]
-[[Category: Hydrolase-rna-dna complex]]

4z7k

From Proteopedia

Revision as of 07:42, 10 May 2023

Crystal structure of CRISPR RNA processing endoribonuclease Cas6b

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox