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4zcm

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Revision as of 07:49, 10 May 2023

Crystal Structure of Escherichia coli GTPase BipA/TypA Complexed with ppGpp

Structural highlights

4zcm is a 2 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

BIPA_ECO27 A 50S ribosomal subunit assembly protein with GTPase activity, required for 50S subunit assembly at low temperatures, may also play a role in translation. Binds GTP and analogs. Binds the 70S ribosome between the 30S and 50S subunits, in a similar position as ribosome-bound EF-G; it contacts a number of ribosomal proteins, both rRNAs and the A-site tRNA (By similarity). GTPase that impacts interactions between enteropathogenic E.coli (EPEC) and epithelial cells and also has an effect on motility (PubMed:9622352).[HAMAP-Rule:MF_00849]^[1]

Publication Abstract from PubMed

The translational GTPase BipA regulates the expression of virulence and pathogenicity factors in several eubacteria. BipA-dependent expression of virulence factors occurs under starvation conditions, such as encountered during infection of a host. Under these conditions, BipA associates with the small ribosomal subunit. BipA also has a second function to promote the efficiency of late steps in biogenesis of large ribosomal subunits at low temperatures, presumably while bound to the ribosome. During starvation, the cellular concentration of stress alarmone guanosine-3', 5'-bis pyrophosphate (ppGpp) is increased. This increase allows ppGpp to bind to BipA and switch its binding specificity from ribosomes to small ribosomal subunits. A conformational change of BipA upon ppGpp binding could explain the ppGpp regulation of BipA's binding specificity. Here, we present the structures of the full-length BipA from Escherichia coli in apo, GDP-, and ppGpp-bound forms. The crystal structure and small-angle X-ray scattering data of the protein with bound nucleotides, together with a thermodynamic analysis of the binding of GDP and of ppGpp to BipA, indicates that the ppGpp-bound form of BipA adopts the structure of the GDP form. This suggests furthermore, that the switch in binding preference only occurs when both ppGpp and the small ribosomal subunit are present. This molecular mechanism would allow BipA to interact with both the ribosome and the small ribosomal subunit during stress response.

Structural and Functional Analysis of BipA, a Regulator of Virulence in enteropathogenic Escherichia coli.,Fan H, Hahm J, Diggs S, Perry JJ, Blaha G J Biol Chem. 2015 Jul 10. pii: jbc.M115.659136. PMID:26163516^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Farris M, Grant A, Richardson TB, O'Connor CD. BipA: a tyrosine-phosphorylated GTPase that mediates interactions between enteropathogenic Escherichia coli (EPEC) and epithelial cells. Mol Microbiol. 1998 Apr;28(2):265-79. PMID:9622352 doi:10.1046/j.1365-2958.1998.00793.x
↑ Fan H, Hahm J, Diggs S, Perry JJ, Blaha G. Structural and Functional Analysis of BipA, a Regulator of Virulence in enteropathogenic Escherichia coli. J Biol Chem. 2015 Jul 10. pii: jbc.M115.659136. PMID:26163516 doi:http://dx.doi.org/10.1074/jbc.M115.659136

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4zcm"

@@ Line 3: / Line 3: @@
 <StructureSection load='4zcm' size='340' side='right'caption='[[4zcm]], [[Resolution|resolution]] 3.31&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4zcm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZCM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZCM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4zcm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZCM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZCM FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G4P:GUANOSINE-5,3-TETRAPHOSPHATE'>G4P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G4P:GUANOSINE-5,3-TETRAPHOSPHATE'>G4P</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zci|4zci]], [[4zck|4zck]], [[4zcl|4zcl]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zcm OCA], [https://pdbe.org/4zcm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zcm RCSB], [https://www.ebi.ac.uk/pdbsum/4zcm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zcm ProSAT]</span></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">typA, bipA, yihK, b3871, JW5571 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zcm OCA], [http://pdbe.org/4zcm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zcm RCSB], [http://www.ebi.ac.uk/pdbsum/4zcm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zcm ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/TYPA_ECOLI TYPA_ECOLI]] Probably interacts with the ribosomes in a GTP dependent manner. GTPase that mediates interactions between enteropathogenic E.coli (EPEC) and epithelial cells.
+[https://www.uniprot.org/uniprot/BIPA_ECO27 BIPA_ECO27] A 50S ribosomal subunit assembly protein with GTPase activity, required for 50S subunit assembly at low temperatures, may also play a role in translation. Binds GTP and analogs. Binds the 70S ribosome between the 30S and 50S subunits, in a similar position as ribosome-bound EF-G; it contacts a number of ribosomal proteins, both rRNAs and the A-site tRNA (By similarity). GTPase that impacts interactions between enteropathogenic E.coli (EPEC) and epithelial cells and also has an effect on motility (PubMed:9622352).[HAMAP-Rule:MF_00849]<ref>PMID:9622352</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 20: @@
 ==See Also==
-*[[GTP-binding protein|GTP-binding protein]]
+*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Ecoli]]
+[[Category: Escherichia coli K-12]]
 [[Category: Large Structures]]
-[[Category: Blaha, G]]
+[[Category: Blaha G]]
-[[Category: Diggs, S]]
+[[Category: Diggs S]]
-[[Category: Fan, H T]]
+[[Category: Fan HT]]
-[[Category: Hahm, J]]
+[[Category: Hahm J]]
-[[Category: Bipa]]
-[[Category: Gtp-binding protein]]
-[[Category: Gtpase]]
-[[Category: Nucleotide]]

4zcm

From Proteopedia

Revision as of 07:49, 10 May 2023

Crystal Structure of Escherichia coli GTPase BipA/TypA Complexed with ppGpp

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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