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4ziz

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Revision as of 07:29, 18 May 2023

Serial Femtosecond Crystallography of Soluble Proteins in Lipidic Cubic Phase (C-Phycocyanin from T. elongatus)

Structural highlights

4ziz is a 2 chain structure with sequence from Thermosynechococcus vestitus BP-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PHCA_THEVB Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex (phycobilisome, PBS). Phycocyanin is the major phycobiliprotein in the PBS rod.

Publication Abstract from PubMed

Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP-SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP-SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.

Serial femtosecond crystallography of soluble proteins in lipidic cubic phase.,Fromme R, Ishchenko A, Metz M, Chowdhury SR, Basu S, Boutet S, Fromme P, White TA, Barty A, Spence JC, Weierstall U, Liu W, Cherezov V IUCrJ. 2015 Aug 4;2(Pt 5):545-51. doi: 10.1107/S2052252515013160. eCollection, 2015 Sep 1. PMID:26306196^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fromme R, Ishchenko A, Metz M, Chowdhury SR, Basu S, Boutet S, Fromme P, White TA, Barty A, Spence JC, Weierstall U, Liu W, Cherezov V. Serial femtosecond crystallography of soluble proteins in lipidic cubic phase. IUCrJ. 2015 Aug 4;2(Pt 5):545-51. doi: 10.1107/S2052252515013160. eCollection, 2015 Sep 1. PMID:26306196 doi:http://dx.doi.org/10.1107/S2052252515013160

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ziz"

@@ Line 3: / Line 3: @@
 <StructureSection load='4ziz' size='340' side='right'caption='[[4ziz]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ziz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus Thermosynechococcus elongatus] and [http://en.wikipedia.org/wiki/Thermosynechococcus_elongatus_(strain_bp-1) Thermosynechococcus elongatus (strain bp-1)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZIZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZIZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ziz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZIZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZIZ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene>, <scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene></td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ziz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ziz OCA], [https://pdbe.org/4ziz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ziz RCSB], [https://www.ebi.ac.uk/pdbsum/4ziz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ziz ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ziz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ziz OCA], [http://pdbe.org/4ziz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ziz RCSB], [http://www.ebi.ac.uk/pdbsum/4ziz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ziz ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PHCA_THEEB PHCA_THEEB]] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex. [[http://www.uniprot.org/uniprot/PHCB_THEEB PHCB_THEEB]] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex.
+[https://www.uniprot.org/uniprot/PHCA_THEVB PHCA_THEVB] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex (phycobilisome, PBS). Phycocyanin is the major phycobiliprotein in the PBS rod.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 27: / Line 26: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Thermosynechococcus elongatus]]
+[[Category: Thermosynechococcus vestitus BP-1]]
-[[Category: Barty, A]]
+[[Category: Barty A]]
-[[Category: Basu, S]]
+[[Category: Basu S]]
-[[Category: Boutet, S]]
+[[Category: Boutet S]]
-[[Category: Cherezov, V]]
+[[Category: Cherezov V]]
-[[Category: Fromme, P]]
+[[Category: Fromme P]]
-[[Category: Fromme, R]]
+[[Category: Fromme R]]
-[[Category: Ishchenko, A]]
+[[Category: Ishchenko A]]
-[[Category: Liu, W]]
+[[Category: Liu W]]
-[[Category: Metz, M]]
+[[Category: Metz M]]
-[[Category: Roy-Chowdhury, S]]
+[[Category: Roy-Chowdhury S]]
-[[Category: Spence, J C.H]]
+[[Category: Spence JCH]]
-[[Category: Weierstall, U]]
+[[Category: Weierstall U]]
-[[Category: White, T A]]
+[[Category: White TA]]
-[[Category: Electron transport]]
-[[Category: Free electron laser]]
-[[Category: Lipidic cubic phase]]
-[[Category: Phycocyanin]]

4ziz

From Proteopedia

Revision as of 07:29, 18 May 2023

Serial Femtosecond Crystallography of Soluble Proteins in Lipidic Cubic Phase (C-Phycocyanin from T. elongatus)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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