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5a6g

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Revision as of 04:41, 25 May 2023

Cryo-EM structure of the Slo2.2 Na-activated K channel

5a6g, resolution 4.50Å

Structural highlights

5a6g is a 2 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Experimental data:	Check to display Experimental Data
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KCNT1_CHICK Generates outwardly rectifying currents that are suppressed by elevation of intracellular calcium.

Publication Abstract from PubMed

Na+-activated K+ channels are members of the Slo family of large conductance K+ channels that are widely expressed in the brain, where their opening regulates neuronal excitability. These channels fulfil a number of biological roles and have intriguing biophysical properties, including conductance levels that are ten times those of most other K+ channels and gating sensitivity to intracellular Na+. Here we present the structure of a complete Na+-activated K+ channel, chicken Slo2.2, in the Na+-free state, determined by cryo-electron microscopy at a nominal resolution of 4.5 angstroms. The channel is composed of a large cytoplasmic gating ring, in which resides the Na+-binding site and a transmembrane domain that closely resembles voltage-gated K+ channels. In the structure, the cytoplasmic domain adopts a closed conformation and the ion conduction pore is also closed. The structure reveals features that can explain the unusually high conductance of Slo channels and how contraction of the cytoplasmic gating ring closes the pore.

Cryo-electron microscopy structure of the Slo2.2 Na-activated K channel.,Hite RK, Yuan P, Li Z, Hsuing Y, Walz T, MacKinnon R Nature. 2015 Oct 5. doi: 10.1038/nature14958. PMID:26436452^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hite RK, Yuan P, Li Z, Hsuing Y, Walz T, MacKinnon R. Cryo-electron microscopy structure of the Slo2.2 Na-activated K channel. Nature. 2015 Oct 5. doi: 10.1038/nature14958. PMID:26436452 doi:http://dx.doi.org/10.1038/nature14958

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5a6g"

@@ Line 3: / Line 3: @@
 <SX load='5a6g' size='340' side='right' viewer='molstar' caption='[[5a6g]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5a6g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A6G OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5A6G FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5a6g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A6G FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a6g OCA], [https://pdbe.org/5a6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a6g RCSB], [https://www.ebi.ac.uk/pdbsum/5a6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a6g ProSAT]</span></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a6f|5a6f]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5a6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a6g OCA], [http://pdbe.org/5a6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a6g RCSB], [http://www.ebi.ac.uk/pdbsum/5a6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a6g ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/KCNT1_CHICK KCNT1_CHICK]] Generates outwardly rectifying currents that are suppressed by elevation of intracellular calcium.
+[https://www.uniprot.org/uniprot/KCNT1_CHICK KCNT1_CHICK] Generates outwardly rectifying currents that are suppressed by elevation of intracellular calcium.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 26: / Line 24: @@
 __TOC__
 </SX>
-[[Category: Chick]]
+[[Category: Gallus gallus]]
 [[Category: Large Structures]]
-[[Category: Hite, R K]]
+[[Category: Hite RK]]
-[[Category: Hsuing, Y]]
+[[Category: Hsuing Y]]
-[[Category: Li, Z]]
+[[Category: Li Z]]
-[[Category: MacKinnon, R]]
+[[Category: MacKinnon R]]
-[[Category: Walz, T]]
+[[Category: Walz T]]
-[[Category: Yuan, P]]
+[[Category: Yuan P]]
-[[Category: Ion channel]]
-[[Category: Potassium channel]]
-[[Category: Transport]]

5a6g

From Proteopedia

Revision as of 04:41, 25 May 2023

Cryo-EM structure of the Slo2.2 Na-activated K channel

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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