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7xz3
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the Type I-B CRISPR-associated protein, Csh2 from Thermobaculum terrenum== | |
| + | <StructureSection load='7xz3' size='340' side='right'caption='[[7xz3]], [[Resolution|resolution]] 1.89Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7xz3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermobaculum_terrenum Thermobaculum terrenum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7XZ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7XZ3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7xz3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7xz3 OCA], [https://pdbe.org/7xz3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7xz3 RCSB], [https://www.ebi.ac.uk/pdbsum/7xz3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7xz3 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/D1CHV0_THET1 D1CHV0_THET1] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Clustered regularly interspaced short palindromic repeats (CRISPR) in many prokaryotes functions as an adaptive immune system against mobile genetic elements. A heterologous ribonucleoprotein silencing complex composed of CRISPR-associated (Cas) proteins and a CRISPR RNA (crRNA) neutralizes the incoming mobile genetic elements. The type I and III silencing complexes commonly include a protein-helical backbone of several copies of identical subunits, for example, Cas7 in the type I silencing complex. In this study, we structurally characterized type I-B Cas7 (Csh2 from Thermobaculum terrenum; TterCsh2). The revealed crystal structure of TterCsh2 shows a typical glove-like architecture of Cas7, which consists of a palm, a thumb, and a finger domain. Csh2 proteins have 5 conserved sequence motifs that are arranged to form a presumable crRNA-binding site in the TterCsh2 structure. This crRNA binding site of TterCsh2 is structurally and potentially comparable to those observed in helix-forming Cas7 structures in other sub-types. Analysis of the reported Cas7 structures and their sequences suggests that Cas7s can be divided into at least two sub-classes. These data will broaden our understanding on the Cascade complex of CRISPR/Cas systems. | ||
| - | + | Structural characterization of the type I-B CRISPR Cas7 from Thermobaculum terrenum.,Seo PW, Gu DH, Kim JW, Kim JH, Park SY, Kim JS Biochim Biophys Acta Proteins Proteom. 2023 May 1;1871(3):140900. doi: , 10.1016/j.bbapap.2023.140900. Epub 2023 Jan 20. PMID:36682394<ref>PMID:36682394</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 7xz3" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Thermobaculum terrenum]] | ||
| + | [[Category: Gu DH]] | ||
| + | [[Category: Kim JS]] | ||
| + | [[Category: Park SY]] | ||
| + | [[Category: Seo PW]] | ||
Revision as of 05:35, 31 May 2023
Crystal structure of the Type I-B CRISPR-associated protein, Csh2 from Thermobaculum terrenum
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