This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

5ap8

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 06:21, 31 May 2023

Structure of the SAM-dependent rRNA:acp-transferase Tsr3 from S. solfataricus

Structural highlights

5ap8 is a 3 chain structure with sequence from Saccharolobus solfataricus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TSR3_SACS2 Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi).[HAMAP-Rule:MF_01116]

Publication Abstract from PubMed

The chemically most complex modification in eukaryotic rRNA is the conserved hypermodified nucleotide N1-methyl-N3-aminocarboxypropyl-pseudouridine (m1acp3Psi) located next to the P-site tRNA on the small subunit 18S rRNA. While S-adenosylmethionine was identified as the source of the aminocarboxypropyl (acp) group more than 40 years ago the enzyme catalyzing the acp transfer remained elusive. Here we identify the cytoplasmic ribosome biogenesis protein Tsr3 as the responsible enzyme in yeast and human cells. In functionally impaired Tsr3-mutants, a reduced level of acp modification directly correlates with increased 20S pre-rRNA accumulation. The crystal structure of archaeal Tsr3 homologs revealed the same fold as in SPOUT-class RNA-methyltransferases but a distinct SAM binding mode. This unique SAM binding mode explains why Tsr3 transfers the acp and not the methyl group of SAM to its substrate. Structurally, Tsr3 therefore represents a novel class of acp transferase enzymes.

Ribosome biogenesis factor Tsr3 is the aminocarboxypropyl transferase responsible for 18S rRNA hypermodification in yeast and humans.,Meyer B, Wurm JP, Sharma S, Immer C, Pogoryelov D, Kotter P, Lafontaine DL, Wohnert J, Entian KD Nucleic Acids Res. 2016 Apr 15. pii: gkw244. PMID:27084949^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Meyer B, Wurm JP, Sharma S, Immer C, Pogoryelov D, Kotter P, Lafontaine DL, Wohnert J, Entian KD. Ribosome biogenesis factor Tsr3 is the aminocarboxypropyl transferase responsible for 18S rRNA hypermodification in yeast and humans. Nucleic Acids Res. 2016 Apr 15. pii: gkw244. PMID:27084949 doi:http://dx.doi.org/10.1093/nar/gkw244

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ap8"

@@ Line 3: / Line 3: @@
 <StructureSection load='5ap8' size='340' side='right'caption='[[5ap8]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ap8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35091 Atcc 35091]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AP8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AP8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ap8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AP8 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5apg|5apg]]</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ap8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ap8 OCA], [https://pdbe.org/5ap8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ap8 RCSB], [https://www.ebi.ac.uk/pdbsum/5ap8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ap8 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ap8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ap8 OCA], [http://pdbe.org/5ap8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ap8 RCSB], [http://www.ebi.ac.uk/pdbsum/5ap8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ap8 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/TSR3_SULSO TSR3_SULSO]] Probable pre-rRNA processing protein involved in ribosome biogenesis.[HAMAP-Rule:MF_01116]
+[https://www.uniprot.org/uniprot/TSR3_SACS2 TSR3_SACS2] Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi).[HAMAP-Rule:MF_01116]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 35091]]
 [[Category: Large Structures]]
-[[Category: Entian, K D]]
+[[Category: Saccharolobus solfataricus]]
-[[Category: Immer, C]]
+[[Category: Entian K-D]]
-[[Category: Koetter, P]]
+[[Category: Immer C]]
-[[Category: Meyer, B]]
+[[Category: Koetter P]]
-[[Category: Pogoryelov, D]]
+[[Category: Meyer B]]
-[[Category: Woehnert, J]]
+[[Category: Pogoryelov D]]
-[[Category: Wurm, J P]]
+[[Category: Woehnert J]]
-[[Category: Pseudouridine]]
+[[Category: Wurm JP]]
-[[Category: Rrna]]
-[[Category: S-adenosylmethionine dependent 3-amino-3-carboxypropyl transferase]]
-[[Category: Sam]]
-[[Category: Spout-fold]]
-[[Category: Transferase]]

5ap8

From Proteopedia

Revision as of 06:21, 31 May 2023

Structure of the SAM-dependent rRNA:acp-transferase Tsr3 from S. solfataricus

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox