1l2h
From Proteopedia
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| - | + | {{STRUCTURE_1l2h| PDB=1l2h | SCENE= }} | |
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'''Crystal structure of Interleukin 1-beta F42W/W120F mutant''' | '''Crystal structure of Interleukin 1-beta F42W/W120F mutant''' | ||
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[[Category: Wilson, I A.]] | [[Category: Wilson, I A.]] | ||
[[Category: Yeates, T O.]] | [[Category: Yeates, T O.]] | ||
| - | [[Category: | + | [[Category: Beta-trefoil]] |
| - | [[Category: | + | [[Category: F42w/w120f double mutant]] |
| - | [[Category: | + | [[Category: Hemihedral twinning]] |
| - | [[Category: | + | [[Category: Interleukin-1 beta]] |
| - | [[Category: | + | [[Category: Mad phasing]] |
| - | [[Category: | + | [[Category: Protein folding]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:27:46 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:27, 2 May 2008
Crystal structure of Interleukin 1-beta F42W/W120F mutant
Overview
The crystal structure at 1.54 A resolution of a double mutant of interleukin-1beta (F42W/W120F), a cytokine secreted by macrophages, was determined by multiple-wavelength anomalous dispersion (MAD) using data from highly twinned selenomethionine-modified crystals. The space group is P4(3), with unit-cell parameters a = b = 53.9, c = 77.4 A. Self-rotation function analysis and various intensity statistics revealed the presence of merohedral twinning in crystals of both the native (twinning fraction alpha approximately 0.35) and SeMet (alpha approximately 0.40) forms. Structure determination and refinement are discussed with emphasis on the possible reasons for successful phasing using untreated twinned MAD data.
About this Structure
1L2H is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Use of multiple anomalous dispersion to phase highly merohedrally twinned crystals of interleukin-1beta., Rudolph MG, Kelker MS, Schneider TR, Yeates TO, Oseroff V, Heidary DK, Jennings PA, Wilson IA, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):290-8. Epub 2003, Jan 23. PMID:12554939 Page seeded by OCA on Fri May 2 23:27:46 2008
