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2mjk

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Nmr structure of hen egg beta-defensin gallin (chicken ovo-defensin)

Structural highlights

2mjk is a 1 chain structure with sequence from Gallus gallus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

D5GR55_CHICK

Publication Abstract from PubMed

Gallin is a 41-residue protein, first identified as a minor component of hen egg white, and found to be antimicrobial against Escherichia coli. Gallin may participate in the protection of the embryo during its development in the egg. Its sequence is related to antimicrobial beta-defensin peptides. In the present study, gallin was chemically synthesized 1) to further investigate its antimicrobial spectrum, and 2) to solve its 3D NMR structure, and thus gain insight into structure-function relationships, a prerequisite to understand its mode(s) of action. Antibacterial assays confirmed that gallin was active against Escherichia coli, but no additional antibacterial activity was observed against the other Gram-positive or Gram-negative bacteria tested. The 3D structure of gallin, which is the first ovo-defensin structure to have been solved to date, displays a new five-stranded arrangement. The gallin 3D fold contains the three-stranded antiparallel beta-sheet and the disulfide bridge array typical of vertebrate beta-defensins. Gallin can therefore be unambiguously classified as a beta-defensin. However, an additional short two-stranded beta-sheet reveals that gallin, and presumably the other ovo-defensins, form a new structural sub-family of beta-defensins. Moreover, gallin and the other ovo-defensins calculated by homology modelling exhibit atypical hydrophobic surface properties, compared with the already known vertebrate beta-defensins. These specific structural features of gallin might be related to its restricted activity against E. coli, and/or to other yet unknown functions. This work provides initial understanding of a critical sequence-structure-function relationship for the ovo-defensin family.

3D NMR structure of hen egg gallin (chicken ovo-defensin) reveals a new variation of the beta-defensin fold.,Herve V, Meudal H, Labas V, Rehault Godbert S, Gautron J, Berges M, Guyot N, Delmas AF, Nys Y, Landon C J Biol Chem. 2014 Jan 17. PMID:24443564^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Herve V, Meudal H, Labas V, Rehault Godbert S, Gautron J, Berges M, Guyot N, Delmas AF, Nys Y, Landon C. 3D NMR structure of hen egg gallin (chicken ovo-defensin) reveals a new variation of the beta-defensin fold. J Biol Chem. 2014 Jan 17. PMID:24443564 doi:http://dx.doi.org/10.1074/jbc.M113.507046

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2mjk"

Categories: Gallus gallus | Large Structures | Landon C | Meudal H

@@ Line 1: / Line 1: @@
 ==Nmr structure of hen egg beta-defensin gallin (chicken ovo-defensin)==
-<StructureSection load='2mjk' size='340' side='right' caption='[[2mjk]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+<StructureSection load='2mjk' size='340' side='right'caption='[[2mjk]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2mjk]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MJK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2mjk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MJK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MJK FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjk OCA], [http://pdbe.org/2mjk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mjk RCSB], [http://www.ebi.ac.uk/pdbsum/2mjk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mjk ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mjk OCA], [https://pdbe.org/2mjk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mjk RCSB], [https://www.ebi.ac.uk/pdbsum/2mjk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mjk ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/D5GR55_CHICK D5GR55_CHICK]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Landon, C]]
+[[Category: Gallus gallus]]
-[[Category: Meudal, H]]
+[[Category: Large Structures]]
-[[Category: Antimicrobial protein]]
+[[Category: Landon C]]
+[[Category: Meudal H]]

2mjk

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Current revision

Nmr structure of hen egg beta-defensin gallin (chicken ovo-defensin)

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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