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| | ==Solution structure of latherin== | | ==Solution structure of latherin== |
| - | <StructureSection load='3zpm' size='340' side='right'caption='[[3zpm]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='3zpm' size='340' side='right'caption='[[3zpm]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3zpm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZPM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZPM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3zpm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZPM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZPM FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zpm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zpm OCA], [http://pdbe.org/3zpm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zpm RCSB], [http://www.ebi.ac.uk/pdbsum/3zpm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zpm ProSAT]</span></td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zpm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zpm OCA], [https://pdbe.org/3zpm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zpm RCSB], [https://www.ebi.ac.uk/pdbsum/3zpm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zpm ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LATH_HORSE LATH_HORSE]] Major protein in sweat, has surfactant properties. Has a role in temperature regulation by having a capacity to make hydrophobic surfaces wettable and so can function in promoting spreading and evaporation of sweat.<ref>PMID:3753435</ref> | + | [https://www.uniprot.org/uniprot/LATH_HORSE LATH_HORSE] Major protein in sweat, has surfactant properties. Has a role in temperature regulation by having a capacity to make hydrophobic surfaces wettable and so can function in promoting spreading and evaporation of sweat.<ref>PMID:3753435</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Equus caballus]] | | [[Category: Equus caballus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cooper, A]] | + | [[Category: Cooper A]] |
| - | [[Category: Kennedy, M W]] | + | [[Category: Kennedy MW]] |
| - | [[Category: MacDonald, R E]] | + | [[Category: MacDonald RE]] |
| - | [[Category: Smith, B O]] | + | [[Category: Smith BO]] |
| - | [[Category: Vance, S J]] | + | [[Category: Vance SJ]] |
| - | [[Category: Bpi]]
| + | |
| - | [[Category: Plunc]]
| + | |
| - | [[Category: Surfactant protein]]
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| Structural highlights
Function
LATH_HORSE Major protein in sweat, has surfactant properties. Has a role in temperature regulation by having a capacity to make hydrophobic surfaces wettable and so can function in promoting spreading and evaporation of sweat.[1]
Publication Abstract from PubMed
Latherin is a highly surface-active allergen protein found in the sweat and saliva of horses and other equids. Its surfactant activity is intrinsic to the protein in its native form, and is manifest without associated lipids or glycosylation. Latherin probably functions as a wetting agent in evaporative cooling in horses, but it may also assist in mastication of fibrous food as well as inhibition of microbial biofilms. It is a member of the PLUNC family of proteins abundant in the oral cavity and saliva of mammals, one of which has also been shown to be a surfactant and capable of disrupting microbial biofilms. How these proteins work as surfactants while remaining soluble and cell membrane-compatible is not known. Nor have their structures previously been reported. We have used protein nuclear magnetic resonance spectroscopy to determine the conformation and dynamics of latherin in aqueous solution. The protein is a monomer in solution with a slightly curved cylindrical structure exhibiting a 'super-roll' motif comprising a four-stranded anti-parallel beta-sheet and two opposing alpha-helices which twist along the long axis of the cylinder. One end of the molecule has prominent, flexible loops that contain a number of apolar amino acid side chains. This, together with previous biophysical observations, leads us to a plausible mechanism for surfactant activity in which the molecule is first localized to the non-polar interface via these loops, and then unfolds and flattens to expose its hydrophobic interior to the air or non-polar surface. Intrinsically surface-active proteins are relatively rare in nature, and this is the first structure of such a protein from mammals to be reported. Both its conformation and proposed method of action are different from other, non-mammalian surfactant proteins investigated so far.
The structure of latherin, a surfactant allergen protein from horse sweat and saliva.,Vance SJ, McDonald RE, Cooper A, Smith BO, Kennedy MW J R Soc Interface. 2013 Jun 19;10(85):20130453. doi: 10.1098/rsif.2013.0453., Print 2013 Aug. PMID:23782536[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Beeley JG, Eason R, Snow DH. Isolation and characterization of latherin, a surface-active protein from horse sweat. Biochem J. 1986 May 1;235(3):645-50. PMID:3753435
- ↑ Vance SJ, McDonald RE, Cooper A, Smith BO, Kennedy MW. The structure of latherin, a surfactant allergen protein from horse sweat and saliva. J R Soc Interface. 2013 Jun 19;10(85):20130453. doi: 10.1098/rsif.2013.0453., Print 2013 Aug. PMID:23782536 doi:10.1098/rsif.2013.0453
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