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5joj

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Calcium-loaded EF-hand domain of L-plastin

Structural highlights

5joj is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PLSL_HUMAN Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.^[1] ^[2]

Publication Abstract from PubMed

L-plastin is a calcium-regulated actin-bundling protein that is expressed in cells of hematopoietic origin and in most metastatic cancer cells. These cell types are mobile and require the constant remodeling of their actin cytoskeleton, where L-plastin bundles filamentous actin. The calcium-dependent regulation of the actin-bundling activity of L-plastin is not well understood. We have used NMR spectroscopy to determine the solution structure of the EF-hand calcium-sensor headpiece domain. Unexpectedly, this domain does not bind directly to the four CH-domains of L-plastin. A novel switch helix is present immediately after the calcium-binding region and it binds tightly to the EF-hand motifs in the presence of calcium. We demonstrate that this switch helix plays a major role during actin-bundling. Moreover a peptide that competitively inhibits the association between the EF-hand motifs and the switch helix was shown to deregulate the actin-bundling activity of L-plastin. Overall, these findings may help to develop new drugs that target the L-plastin headpiece and interfere in the metastatic activity of cancer cells.

The Calcium-Dependent Switch Helix of L-Plastin Regulates Actin Bundling.,Ishida H, Jensen KV, Woodman AG, Hyndman ME, Vogel HJ Sci Rep. 2017 Feb 1;7:40662. doi: 10.1038/srep40662. PMID:28145401^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Janji B, Giganti A, De Corte V, Catillon M, Bruyneel E, Lentz D, Plastino J, Gettemans J, Friederich E. Phosphorylation on Ser5 increases the F-actin-binding activity of L-plastin and promotes its targeting to sites of actin assembly in cells. J Cell Sci. 2006 May 1;119(Pt 9):1947-60. PMID:16636079 doi:http://dx.doi.org/10.1242/jcs.02874
↑ Wabnitz GH, Kocher T, Lohneis P, Stober C, Konstandin MH, Funk B, Sester U, Wilm M, Klemke M, Samstag Y. Costimulation induced phosphorylation of L-plastin facilitates surface transport of the T cell activation molecules CD69 and CD25. Eur J Immunol. 2007 Mar;37(3):649-62. PMID:17294403 doi:http://dx.doi.org/10.1002/eji.200636320
↑ Ishida H, Jensen KV, Woodman AG, Hyndman ME, Vogel HJ. The Calcium-Dependent Switch Helix of L-Plastin Regulates Actin Bundling. Sci Rep. 2017 Feb 1;7:40662. doi: 10.1038/srep40662. PMID:28145401 doi:http://dx.doi.org/10.1038/srep40662

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5joj"

@@ Line 1: / Line 1: @@
 ==Calcium-loaded EF-hand domain of L-plastin==
-<StructureSection load='5joj' size='340' side='right' caption='[[5joj]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''>
+<StructureSection load='5joj' size='340' side='right'caption='[[5joj]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5joj]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JOJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JOJ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5joj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JOJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JOJ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5jol|5jol]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5joj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5joj OCA], [https://pdbe.org/5joj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5joj RCSB], [https://www.ebi.ac.uk/pdbsum/5joj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5joj ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5joj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5joj OCA], [http://pdbe.org/5joj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5joj RCSB], [http://www.ebi.ac.uk/pdbsum/5joj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5joj ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/PLSL_HUMAN PLSL_HUMAN]] Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.<ref>PMID:16636079</ref> <ref>PMID:17294403</ref>
+[https://www.uniprot.org/uniprot/PLSL_HUMAN PLSL_HUMAN] Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.<ref>PMID:16636079</ref> <ref>PMID:17294403</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Hyndman, M E]]
+[[Category: Homo sapiens]]
-[[Category: Ishida, H]]
+[[Category: Large Structures]]
-[[Category: Jensen, K V]]
+[[Category: Hyndman ME]]
-[[Category: Vogel, H J]]
+[[Category: Ishida H]]
-[[Category: Woodman, G W]]
+[[Category: Jensen KV]]
-[[Category: Calcium-binding]]
+[[Category: Vogel HJ]]
-[[Category: Ef-hand]]
+[[Category: Woodman GW]]
-[[Category: L-plastin]]
-[[Category: Metal binding protein]]

5joj

From Proteopedia

Current revision

Calcium-loaded EF-hand domain of L-plastin

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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