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| | ==Solution structure of the IreB homodimer== | | ==Solution structure of the IreB homodimer== |
| - | <StructureSection load='5us5' size='340' side='right' caption='[[5us5]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5us5' size='340' side='right'caption='[[5us5]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5us5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Entfa Entfa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5US5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5US5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5us5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis_V583 Enterococcus faecalis V583]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5US5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5US5 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EF_1202 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=226185 ENTFA])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5us5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5us5 OCA], [https://pdbe.org/5us5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5us5 RCSB], [https://www.ebi.ac.uk/pdbsum/5us5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5us5 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5us5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5us5 OCA], [http://pdbe.org/5us5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5us5 RCSB], [http://www.ebi.ac.uk/pdbsum/5us5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5us5 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Y1202_ENTFA Y1202_ENTFA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Entfa]] | + | [[Category: Enterococcus faecalis V583]] |
| - | [[Category: Structural genomic]] | + | [[Category: Large Structures]] |
| - | [[Category: Kristich, C J]] | + | [[Category: Kristich CJ]] |
| - | [[Category: Lytle, B L]] | + | [[Category: Lytle BL]] |
| - | [[Category: Peterson, F C]] | + | [[Category: Peterson FC]] |
| - | [[Category: Volkman, B F]] | + | [[Category: Volkman BF]] |
| - | [[Category: Cesg]]
| + | |
| - | [[Category: Dimer]]
| + | |
| - | [[Category: Ireb]]
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| - | [[Category: PSI, Protein structure initiative]]
| + | |
| - | [[Category: Structure from molmol]]
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| Structural highlights
Function
Y1202_ENTFA
Publication Abstract from PubMed
Enterococcus faecalis, a leading cause of hospital-acquired infections, exhibits intrinsic resistance to most cephalosporins, which are antibiotics in the beta-lactam family that target cell-wall biosynthesis. A comprehensive understanding of the underlying genetic and biochemical mechanisms of cephalosporin resistance in E. faecalis is lacking. We previously determined that a transmembrane serine/threonine kinase (IreK) and its cognate phosphatase (IreP) reciprocally regulate cephalosporin resistance in E. faecalis, dependent on the kinase activity of IreK. Other than IreK itself, thus far the only known substrate for reversible phosphorylation by IreK and IreP is IreB, a small protein of unknown function that is well conserved in low-GC Gram-positive bacteria. We previously showed that IreB acts as a negative regulator of cephalosporin resistance in E. faecalis. However, the biochemical mechanism by which IreB modulates cephalosporin resistance remains unknown. As a next step toward an understanding of the mechanism by which IreB regulates resistance, we initiated a structure-function study on IreB. The NMR solution structure of IreB was determined, revealing that IreB adopts a unique fold and forms a dimer in vitro. Dimerization of IreB was confirmed in vivo. Substitutions at the dimer interface impaired IreB function and stability in vivo, indicating that dimerization is functionally important for the biological activity of IreB. Hence, these studies provide new insights into the structure and function of a widely conserved protein of unknown function that is an important regulator of antimicrobial resistance in E. faecalis.
Structure and Dimerization of IreB, a Negative Regulator of Cephalosporin Resistance in Enterococcus faecalis.,Hall CL, Lytle BL, Jensen D, Hoff JS, Peterson FC, Volkman BF, Kristich CJ J Mol Biol. 2017 Jul 21;429(15):2324-2336. doi: 10.1016/j.jmb.2017.05.019. Epub, 2017 May 24. PMID:28551334[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hall CL, Lytle BL, Jensen D, Hoff JS, Peterson FC, Volkman BF, Kristich CJ. Structure and Dimerization of IreB, a Negative Regulator of Cephalosporin Resistance in Enterococcus faecalis. J Mol Biol. 2017 Jul 21;429(15):2324-2336. doi: 10.1016/j.jmb.2017.05.019. Epub, 2017 May 24. PMID:28551334 doi:http://dx.doi.org/10.1016/j.jmb.2017.05.019
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