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5zcn
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5zcn is ON HOLD until Paper Publication Authors: Hemmi, H., Kodani, S., Miyake, Y., Kaweewan, I., Nakagawa, H. Description: Solution NMR structure ...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution NMR structure of a new lasso peptide brevunsin== | |
| + | <StructureSection load='5zcn' size='340' side='right'caption='[[5zcn]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5zcn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brevundimonas_diminuta Brevundimonas diminuta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZCN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZCN FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zcn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zcn OCA], [https://pdbe.org/5zcn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zcn RCSB], [https://www.ebi.ac.uk/pdbsum/5zcn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zcn ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A381AKI5_BREDI A0A381AKI5_BREDI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A shuttle vector pHSG396Sp was constructed to perform gene expression using Sphingomonas subterranea as a host. A new lasso peptide biosynthetic gene cluster, derived from Brevundimonas diminuta, was amplified by PCR and integrated to afford a expression vector pHSG396Sp-12697L. The new lasso peptide brevunsin was successfully produced by S. subterranea, harboring the expression vector, with a high production yield (10.2 mg from 1 L culture). The chemical structure of brevunsin was established by NMR and MS/MS experiments. Based on the information obtained from the NOE experiment, the three-dimensional structure of brevunsin was determined, which indicated that brevunsin possessed a typical lasso structure. This expression vector system provides a new heterologous production method for unexplored lasso peptides that are encoded by bacterial genomes. | ||
| - | + | Heterologous production of a new lasso peptide brevunsin in Sphingomonas subterranea.,Kodani S, Hemmi H, Miyake Y, Kaweewan I, Nakagawa H J Ind Microbiol Biotechnol. 2018 Nov;45(11):983-992. doi:, 10.1007/s10295-018-2077-6. Epub 2018 Sep 6. PMID:30191430<ref>PMID:30191430</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5zcn" style="background-color:#fffaf0;"></div> |
| - | [[Category: Hemmi | + | == References == |
| - | [[Category: Kaweewan | + | <references/> |
| - | [[Category: Miyake | + | __TOC__ |
| - | [[Category: Nakagawa | + | </StructureSection> |
| + | [[Category: Brevundimonas diminuta]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Hemmi H]] | ||
| + | [[Category: Kaweewan I]] | ||
| + | [[Category: Kodani S]] | ||
| + | [[Category: Miyake Y]] | ||
| + | [[Category: Nakagawa H]] | ||
Current revision
Solution NMR structure of a new lasso peptide brevunsin
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