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6e3c

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NMR Solution Structure of the Monomeric Form of the Phage L Decoration Protein

Structural highlights

6e3c is a 1 chain structure with sequence from Enterobacteria phage L. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q5C838_9CAUD

Publication Abstract from PubMed

The major coat proteins of dsDNA tailed phages (order Caudovirales) and herpesviruses form capsids by a mechanism that includes active packaging of the dsDNA genome into a precursor procapsid, followed by expansion and stabilization of the capsid. These viruses have evolved diverse strategies to fortify their capsids, such as non-covalent binding of auxiliary 'decoration' (Dec) proteins. The Dec protein from the P22-like phage L has a highly unusual binding strategy that distinguishes between nearly identical three-fold and quasi-three-fold sites of the icosahedral capsid. Cryo-electron microscopy and three-dimensional image reconstruction were employed to determine the structure of native phage L particles. NMR was used to determine the structure/dynamics of Dec in solution. The NMR structure and the cryo-EM density envelope were combined to build a model of the capsid-bound Dec trimer. Key regions that modulate the binding interface were verified by site-directed mutagenesis.

The phage L capsid decoration protein has a novel OB-fold and an unusual capsid binding strategy.,Newcomer RL, Schrad JR, Gilcrease EB, Casjens SR, Feig M, Teschke CM, Alexandrescu AT, Parent KN Elife. 2019 Apr 4;8. pii: 45345. doi: 10.7554/eLife.45345. PMID:30945633^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Newcomer RL, Schrad JR, Gilcrease EB, Casjens SR, Feig M, Teschke CM, Alexandrescu AT, Parent KN. The phage L capsid decoration protein has a novel OB-fold and an unusual capsid binding strategy. Elife. 2019 Apr 4;8. pii: 45345. doi: 10.7554/eLife.45345. PMID:30945633 doi:http://dx.doi.org/10.7554/eLife.45345

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6e3c"

Categories: Enterobacteria phage L | Large Structures | Alexandrescu AT | Newcomer RL | Teschke CM

@@ Line 1: / Line 1: @@
 ==NMR Solution Structure of the Monomeric Form of the Phage L Decoration Protein==
-<StructureSection load='6e3c' size='340' side='right'caption='[[6e3c]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='6e3c' size='340' side='right'caption='[[6e3c]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6e3c]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_l Bacteriophage l]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E3C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6E3C FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6e3c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_L Enterobacteria phage L]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6E3C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6E3C FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dec ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=45441 Bacteriophage L])</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6e3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e3c OCA], [https://pdbe.org/6e3c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6e3c RCSB], [https://www.ebi.ac.uk/pdbsum/6e3c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6e3c ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6e3c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6e3c OCA], [http://pdbe.org/6e3c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6e3c RCSB], [http://www.ebi.ac.uk/pdbsum/6e3c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6e3c ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q5C838_9CAUD Q5C838_9CAUD]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Bacteriophage l]]
+[[Category: Enterobacteria phage L]]
 [[Category: Large Structures]]
-[[Category: Alexandrescu, A T]]
+[[Category: Alexandrescu AT]]
-[[Category: Newcomer, R L]]
+[[Category: Newcomer RL]]
-[[Category: Teschke, C M]]
+[[Category: Teschke CM]]
-[[Category: Cementing]]
-[[Category: Decoration]]
-[[Category: Ob-fold]]
-[[Category: Viral protein]]

6e3c

From Proteopedia

Current revision

NMR Solution Structure of the Monomeric Form of the Phage L Decoration Protein

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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