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6g03

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NMR Solution Structure of yeast TSR2(1-152)

Structural highlights

6g03 is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TSR2_YEAST Required for 20S pre-rRNA processing.^[1]

Publication Abstract from PubMed

Disordered extensions at the termini and short internal insertions distinguish eukaryotic ribosomal proteins (r-proteins) from their anucleated archaeal counterparts. Here, we report an NMR structure of such a eukaryotic-specific segment (ESS) in the r-protein eS26 in complex with the escortin Tsr2. The structure reveals how ESS attracts Tsr2 specifically to importin:eS26 complexes entering the nucleus in order to trigger non-canonical RanGTP-independent disassembly. Tsr2 then sequesters the released eS26 and prevents rebinding to the importin, providing an alternative allosteric mechanism to terminate the process of nuclear import. Notably, a Diamond-Blackfan anemia-associated Tsr2 mutant protein is impaired in binding to ESS, unveiling a critical role for this interaction in human hematopoiesis. We propose that eS26-ESS and Tsr2 are components of a nuclear sorting system that co-evolved with the emergence of the nucleocytoplasmic barrier and transport carriers.

Molecular basis for disassembly of an importin:ribosomal protein complex by the escortin Tsr2.,Schutz S, Michel E, Damberger FF, Oplova M, Pena C, Leitner A, Aebersold R, Allain FH, Panse VG Nat Commun. 2018 Sep 10;9(1):3669. doi: 10.1038/s41467-018-06160-x. PMID:30201955^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Peng WT, Robinson MD, Mnaimneh S, Krogan NJ, Cagney G, Morris Q, Davierwala AP, Grigull J, Yang X, Zhang W, Mitsakakis N, Ryan OW, Datta N, Jojic V, Pal C, Canadien V, Richards D, Beattie B, Wu LF, Altschuler SJ, Roweis S, Frey BJ, Emili A, Greenblatt JF, Hughes TR. A panoramic view of yeast noncoding RNA processing. Cell. 2003 Jun 27;113(7):919-33. PMID:12837249
↑ Schutz S, Michel E, Damberger FF, Oplova M, Pena C, Leitner A, Aebersold R, Allain FH, Panse VG. Molecular basis for disassembly of an importin:ribosomal protein complex by the escortin Tsr2. Nat Commun. 2018 Sep 10;9(1):3669. doi: 10.1038/s41467-018-06160-x. PMID:30201955 doi:http://dx.doi.org/10.1038/s41467-018-06160-x

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6g03"

@@ Line 1: / Line 1: @@
 ==NMR Solution Structure of yeast TSR2(1-152)==
-<StructureSection load='6g03' size='340' side='right' caption='[[6g03]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='6g03' size='340' side='right'caption='[[6g03]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6g03]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G03 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6G03 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6g03]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6G03 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6G03 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6g03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g03 OCA], [http://pdbe.org/6g03 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6g03 RCSB], [http://www.ebi.ac.uk/pdbsum/6g03 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6g03 ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6g03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6g03 OCA], [https://pdbe.org/6g03 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6g03 RCSB], [https://www.ebi.ac.uk/pdbsum/6g03 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6g03 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/TSR2_YEAST TSR2_YEAST]] Required for 20S pre-rRNA processing.<ref>PMID:12837249</ref>
+[https://www.uniprot.org/uniprot/TSR2_YEAST TSR2_YEAST] Required for 20S pre-rRNA processing.<ref>PMID:12837249</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Allain, F H.T]]
+[[Category: Large Structures]]
-[[Category: Damberger, F F]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Michel, E]]
+[[Category: Allain FH-T]]
-[[Category: Panse, V G]]
+[[Category: Damberger FF]]
-[[Category: Schuetz, S]]
+[[Category: Michel E]]
-[[Category: Alpha-helical protein]]
+[[Category: Panse VG]]
-[[Category: Escortin]]
+[[Category: Schuetz S]]
-[[Category: Eukaryotic specific segment]]
-[[Category: Nuclear unloading factor]]
-[[Category: Nucleus]]
-[[Category: Ribosome maturation]]
-[[Category: Transport of ribosomal protein s26]]
-[[Category: Transport protein]]

6g03

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Current revision

NMR Solution Structure of yeast TSR2(1-152)

Structural highlights

Function

Publication Abstract from PubMed

References

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