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| | ==Structural and DNA Binding Properties of Mycobacterial Integration Host Factor mIHF== | | ==Structural and DNA Binding Properties of Mycobacterial Integration Host Factor mIHF== |
| - | <StructureSection load='6tob' size='340' side='right'caption='[[6tob]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='6tob' size='340' side='right'caption='[[6tob]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6tob]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TOB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6TOB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6tob]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TOB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TOB FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mihF, ERS007672_05099, ERS007703_04035 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr> | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tob OCA], [https://pdbe.org/6tob PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tob RCSB], [https://www.ebi.ac.uk/pdbsum/6tob PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tob ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6tob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tob OCA], [http://pdbe.org/6tob PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tob RCSB], [http://www.ebi.ac.uk/pdbsum/6tob PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tob ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/P71658_MYCTU P71658_MYCTU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Herrmann, T]] | + | [[Category: Mycobacterium tuberculosis]] |
| - | [[Category: Transcriptional control. mycobacterial integration host factor - mihf is an alpha-helical protein with two dna-binding site]] | + | [[Category: Herrmann T]] |
| - | [[Category: Dna binding protein]]
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| Structural highlights
Function
P71658_MYCTU
Publication Abstract from PubMed
In bacteria, nucleoid associated proteins (NAPs) take part in active chromosome organization by supercoil management, three-dimensional DNA looping and direct transcriptional control. Mycobacterial integration host factor (mIHF, rv1388) is a NAP restricted to Actinobacteria and essential for survival of the human pathogen Mycobacterium tuberculosis. We show in vitro that DNA binding by mIHF strongly stabilizes the protein and increases its melting temperature. The structure obtained by Nuclear Magnetic Resonance (NMR) spectroscopy characterizes mIHF as a globular protein with a protruding alpha helix and a disordered N-terminus, similar to Streptomyces coelicolor IHF (sIHF). NMR revealed no residues of high flexibility, suggesting that mIHF is a rigid protein overall that does not undergo structural rearrangements. We show that mIHF only binds to double stranded DNA in solution, through two DNA binding sites (DBSs) similar to those identified in the x-ray structure of sIHF. According to Atomic Force Microscopy, mIHF is able to introduce left-handed loops of ca. 100 nm size ( approximately 300 bp) in supercoiled cosmids, thereby unwinding and relaxing the DNA.
Structural and DNA Binding Properties of Mycobacterial Integration Host Factor mIHF.,Odermatt NT, Lelli M, Herrmann T, Abriata LA, Japaridze A, Voilquin H, Singh R, Piton J, Emsley L, Dietler G, Cole ST J Struct Biol. 2019 Dec 14:107434. doi: 10.1016/j.jsb.2019.107434. PMID:31846718[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Odermatt NT, Lelli M, Herrmann T, Abriata LA, Japaridze A, Voilquin H, Singh R, Piton J, Emsley L, Dietler G, Cole ST. Structural and DNA Binding Properties of Mycobacterial Integration Host Factor mIHF. J Struct Biol. 2019 Dec 14:107434. doi: 10.1016/j.jsb.2019.107434. PMID:31846718 doi:http://dx.doi.org/10.1016/j.jsb.2019.107434
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