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6ytc
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6ytc is ON HOLD Authors: Rath, P., Mazur, A., Hiller, S. Description: Solution NMR structure of the isolated NTE domain of BT1762-63 levan transpor...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Solution NMR structure of the isolated NTE domain of BT1762-63 levan transporter== | |
| + | <StructureSection load='6ytc' size='340' side='right'caption='[[6ytc]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6ytc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_thetaiotaomicron Bacteroides thetaiotaomicron]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YTC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YTC FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ytc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ytc OCA], [https://pdbe.org/6ytc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ytc RCSB], [https://www.ebi.ac.uk/pdbsum/6ytc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ytc ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q8A6W3_BACTN Q8A6W3_BACTN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In Bacteroidetes, one of the dominant phyla of the mammalian gut, active uptake of large nutrients across the outer membrane is mediated by SusCD protein complexes via a "pedal bin" transport mechanism. However, many features of SusCD function in glycan uptake remain unclear, including ligand binding, the role of the SusD lid and the size limit for substrate transport. Here we characterise the beta2,6 fructo-oligosaccharide (FOS) importing SusCD from Bacteroides thetaiotaomicron (Bt1762-Bt1763) to shed light on SusCD function. Co-crystal structures reveal residues involved in glycan recognition and suggest that the large binding cavity can accommodate several substrate molecules, each up to ~2.5 kDa in size, a finding supported by native mass spectrometry and isothermal titration calorimetry. Mutational studies in vivo provide functional insights into the key structural features of the SusCD apparatus and cryo-EM of the intact dimeric SusCD complex reveals several distinct states of the transporter, directly visualising the dynamics of the pedal bin transport mechanism. | ||
| - | + | Insights into SusCD-mediated glycan import by a prominent gut symbiont.,Gray DA, White JBR, Oluwole AO, Rath P, Glenwright AJ, Mazur A, Zahn M, Basle A, Morland C, Evans SL, Cartmell A, Robinson CV, Hiller S, Ranson NA, Bolam DN, van den Berg B Nat Commun. 2021 Jan 4;12(1):44. doi: 10.1038/s41467-020-20285-y. PMID:33398001<ref>PMID:33398001</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Hiller | + | <div class="pdbe-citations 6ytc" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacteroides thetaiotaomicron]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Hiller S]] | ||
| + | [[Category: Mazur A]] | ||
| + | [[Category: Rath P]] | ||
Current revision
Solution NMR structure of the isolated NTE domain of BT1762-63 levan transporter
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