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7o5e
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | The | + | ==The structure of an i-motif/duplex junction at neutral pH== |
| + | <StructureSection load='7o5e' size='340' side='right'caption='[[7o5e]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7o5e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O5E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O5E FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DNR:2-DEOXY-N3-PROTONATED+CYTIDINE-5-MONOPHOSPHATE'>DNR</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o5e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o5e OCA], [https://pdbe.org/7o5e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o5e RCSB], [https://www.ebi.ac.uk/pdbsum/7o5e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o5e ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We report here the three-dimensional structure of an i-motif/duplex junction, determined by NMR methods at neutral pH. By including a minor groove tetrad at one side of the C:C(+) stack of a monomeric i-motif, and a stem/loop hairpin at the other side, we have designed stable DNA constructs in which i-DNA and B-DNA regions coexist in a wide range of experimental conditions. This study demonstrates that i- and B-DNA are structurally compatible, giving rise to a distinctive fold with peculiar groove shapes. The effect of different residues at the i-motif/duplex interface has been explored. We also show that these constructs can be adapted to sequences of biological relevance, like that found in the promoter region of the KRAS oncogene. | ||
| - | + | Structure of i-Motif/Duplex Junctions at Neutral pH.,Serrano-Chacon I, Mir B, Escaja N, Gonzalez C J Am Chem Soc. 2021 Aug 25;143(33):12919-12923. doi: 10.1021/jacs.1c04679. Epub, 2021 Aug 9. PMID:34370473<ref>PMID:34370473</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7o5e" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Escaja N]] | ||
| + | [[Category: Gonzalez C]] | ||
| + | [[Category: Mir B]] | ||
| + | [[Category: Serrano-Chacon I]] | ||
Current revision
The structure of an i-motif/duplex junction at neutral pH
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