1l7z
From Proteopedia
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'''Crystal structure of Ca2+/Calmodulin complexed with myristoylated CAP-23/NAP-22 peptide''' | '''Crystal structure of Ca2+/Calmodulin complexed with myristoylated CAP-23/NAP-22 peptide''' | ||
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[[Category: Taniguchi, H.]] | [[Category: Taniguchi, H.]] | ||
[[Category: Yamauchi, E.]] | [[Category: Yamauchi, E.]] | ||
| - | [[Category: | + | [[Category: Calmodulin]] |
| - | [[Category: | + | [[Category: Myristoylation]] |
| - | [[Category: | + | [[Category: Protein-protein interaction]] |
| - | [[Category: | + | [[Category: Riken structural genomics/proteomics initiative]] |
| - | [[Category: | + | [[Category: Rsgi]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:38:52 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 20:38, 2 May 2008
Crystal structure of Ca2+/Calmodulin complexed with myristoylated CAP-23/NAP-22 peptide
Overview
A variety of viral and signal transduction proteins are known to be myristoylated. Although the role of myristoylation in protein-lipid interaction is well established, the involvement of myristoylation in protein-protein interactions is less well understood. CAP-23/NAP-22 is a brain-specific protein kinase C substrate protein that is involved in axon regeneration. Although the protein lacks any canonical calmodulin (CaM)-binding domain, it binds CaM with high affinity. The binding of CAP-23/NAP-22 to CaM is myristoylation dependent and the N-terminal myristoyl group is directly involved in the protein-protein interaction. Here we show the crystal structure of Ca2+-CaM bound to a myristoylated peptide corresponding to the N-terminal domain of CAP-23/NAP-22. The myristoyl moiety of the peptide goes through a hydrophobic tunnel created by the hydrophobic pockets in the N- and C-terminal domains of CaM. In addition to the myristoyl group, several amino-acid residues in the peptide are important for CaM binding. This is a novel mode of binding and is very different from the mechanism of binding in other CaM-target complexes.
About this Structure
1L7Z is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of a myristoylated CAP-23/NAP-22 N-terminal domain complexed with Ca2+/calmodulin., Matsubara M, Nakatsu T, Kato H, Taniguchi H, EMBO J. 2004 Feb 25;23(4):712-8. Epub 2004 Feb 12. PMID:14765114 Page seeded by OCA on Fri May 2 23:38:52 2008
Categories: Homo sapiens | Protein complex | Kato, H. | Matsubara, M. | Nakatsu, T. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Taniguchi, H. | Yamauchi, E. | Calmodulin | Myristoylation | Protein-protein interaction | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic
