This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5co8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (12:19, 14 June 2023) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
-
'''Unreleased structure'''
 
-
The entry 5co8 is ON HOLD
+
==Crystal structure of the Holliday junction-resolving enzyme GEN1 (WT) in complex with product DNA and Mg2+ ion==
 +
<StructureSection load='5co8' size='340' side='right'caption='[[5co8]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[5co8]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CO8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CO8 FirstGlance]. <br>
 +
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5co8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5co8 OCA], [https://pdbe.org/5co8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5co8 RCSB], [https://www.ebi.ac.uk/pdbsum/5co8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5co8 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/G0RYN2_CHATD G0RYN2_CHATD]
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
We present the crystal structure of the junction-resolving enzyme GEN1 bound to DNA at 2.5 A resolution. The structure of the GEN1 protein reveals it to have an elaborated FEN-XPG family fold that is modified for its role in four-way junction resolution. The functional unit in the crystal is a monomer of active GEN1 bound to the product of resolution cleavage, with an extensive DNA binding interface for both helical arms. Within the crystal lattice, a GEN1 dimer interface juxtaposes two products, whereby they can be reconnected into a four-way junction, the structure of which agrees with that determined in solution. The reconnection requires some opening of the DNA structure at the center, in agreement with permanganate probing and 2-aminopurine fluorescence. The structure shows that a relaxation of the DNA structure accompanies cleavage, suggesting how second-strand cleavage is accelerated to ensure productive resolution of the junction.
-
Authors: Liu, Y.J., Freeman, A.D.J., Declais, A.C., Wilson, T.J., Gartner, A., Lilley, D.M.J.
+
Crystal Structure of a Eukaryotic GEN1 Resolving Enzyme Bound to DNA.,Liu Y, Freeman AD, Declais AC, Wilson TJ, Gartner A, Lilley DM Cell Rep. 2015 Dec 22;13(11):2565-75. doi: 10.1016/j.celrep.2015.11.042. Epub, 2015 Dec 10. PMID:26686639<ref>PMID:26686639</ref>
-
Description: Crystal structure of the Holliday junction-resolving enzyme GEN1 (WT) in complex with product DNA and Mg2+ ion
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Unreleased Structures]]
+
</div>
-
[[Category: Gartner, A]]
+
<div class="pdbe-citations 5co8" style="background-color:#fffaf0;"></div>
-
[[Category: Lilley, D.M.J]]
+
== References ==
-
[[Category: Declais, A.C]]
+
<references/>
-
[[Category: Freeman, A.D.J]]
+
__TOC__
-
[[Category: Liu, Y.J]]
+
</StructureSection>
-
[[Category: Wilson, T.J]]
+
[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
 +
[[Category: Large Structures]]
 +
[[Category: Synthetic construct]]
 +
[[Category: Declais AC]]
 +
[[Category: Freeman ADJ]]
 +
[[Category: Gartner A]]
 +
[[Category: Lilley DMJ]]
 +
[[Category: Liu YJ]]
 +
[[Category: Wilson TJ]]

Current revision

Crystal structure of the Holliday junction-resolving enzyme GEN1 (WT) in complex with product DNA and Mg2+ ion

PDB ID 5co8

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5co8"
Personal tools