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5d1z

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IsdB NEAT1 bound by clone D4-10

Structural highlights

5d1z is a 10 chain structure with sequence from Homo sapiens and Staphylococcus aureus subsp. aureus MSSA476. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CDCA2_HUMAN Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates.^[1] ^[2]

Publication Abstract from PubMed

Staphylococcus aureus is both an important pathogen and a human commensal. To explore this ambivalent relationship between host and microbe, we analysed the memory humoral response against IsdB, a protein involved in iron acquisition, in four healthy donors. Here we show that in all donors a heavily biased use of two immunoglobulin heavy chain germlines generated high affinity (pM) antibodies that neutralize the two IsdB NEAT domains, IGHV4-39 for NEAT1 and IGHV1-69 for NEAT2. In contrast to the typical antibody/antigen interactions, the binding is primarily driven by the germline-encoded hydrophobic CDRH-2 motifs of IGHV1-69 and IGHV4-39, with a binding mechanism nearly identical for each antibody derived from different donors. Our results suggest that IGHV1-69 and IGHV4-39, while part of the adaptive immune system, may have evolved under selection pressure to encode a binding motif innately capable of recognizing and neutralizing a structurally conserved protein domain involved in pathogen iron acquisition.

Germline-encoded neutralization of a Staphylococcus aureus virulence factor by the human antibody repertoire.,Yeung YA, Foletti D, Deng X, Abdiche Y, Strop P, Glanville J, Pitts S, Lindquist K, Sundar PD, Sirota M, Hasa-Moreno A, Pham A, Melton Witt J, Ni I, Pons J, Shelton D, Rajpal A, Chaparro-Riggers J Nat Commun. 2016 Nov 18;7:13376. doi: 10.1038/ncomms13376. PMID:27857134^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Trinkle-Mulcahy L, Andersen J, Lam YW, Moorhead G, Mann M, Lamond AI. Repo-Man recruits PP1 gamma to chromatin and is essential for cell viability. J Cell Biol. 2006 Feb 27;172(5):679-92. Epub 2006 Feb 21. PMID:16492807 doi:http://dx.doi.org/10.1083/jcb.200508154
↑ Vagnarelli P, Hudson DF, Ribeiro SA, Trinkle-Mulcahy L, Spence JM, Lai F, Farr CJ, Lamond AI, Earnshaw WC. Condensin and Repo-Man-PP1 co-operate in the regulation of chromosome architecture during mitosis. Nat Cell Biol. 2006 Oct;8(10):1133-42. Epub 2006 Sep 24. PMID:16998479 doi:http://dx.doi.org/ncb1475
↑ Yeung YA, Foletti D, Deng X, Abdiche Y, Strop P, Glanville J, Pitts S, Lindquist K, Sundar PD, Sirota M, Hasa-Moreno A, Pham A, Melton Witt J, Ni I, Pons J, Shelton D, Rajpal A, Chaparro-Riggers J. Germline-encoded neutralization of a Staphylococcus aureus virulence factor by the human antibody repertoire. Nat Commun. 2016 Nov 18;7:13376. doi: 10.1038/ncomms13376. PMID:27857134 doi:http://dx.doi.org/10.1038/ncomms13376

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5d1z"

Categories: Homo sapiens | Large Structures | Staphylococcus aureus subsp. aureus MSSA476 | Deng X

@@ Line 1: / Line 1: @@
 ==IsdB NEAT1 bound by clone D4-10==
-<StructureSection load='5d1z' size='340' side='right' caption='[[5d1z]], [[Resolution|resolution]] 3.17&Aring;' scene=''>
+<StructureSection load='5d1z' size='340' side='right'caption='[[5d1z]], [[Resolution|resolution]] 3.17&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5d1z]] is a 10 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D1Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5D1Z FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5d1z]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_MSSA476 Staphylococcus aureus subsp. aureus MSSA476]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D1Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D1Z FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5d1x|5d1x]], [[5diq|5diq]]</td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d1z OCA], [https://pdbe.org/5d1z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d1z RCSB], [https://www.ebi.ac.uk/pdbsum/5d1z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d1z ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5d1z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d1z OCA], [http://pdbe.org/5d1z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5d1z RCSB], [http://www.ebi.ac.uk/pdbsum/5d1z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5d1z ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ISDB_STAAS ISDB_STAAS]] Seems to function as the primary receptor for hemoglobin since its inactivation inhibits the ability of S.aureus to bind hemoglobin. Binds hemoglobin in a dose-dependent way. Required for S.aureus growth using hemoglobin as the sole iron source. Also required for virulence. IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils (By similarity).
+[https://www.uniprot.org/uniprot/CDCA2_HUMAN CDCA2_HUMAN] Regulator of chromosome structure during mitosis required for condensin-depleted chromosomes to retain their compact architecture through anaphase. Acts by mediating the recruitment of phopsphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates.<ref>PMID:16492807</ref> <ref>PMID:16998479</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 21: @@
 __TOC__
 </StructureSection>
-[[Category: Deng, X]]
+[[Category: Homo sapiens]]
-[[Category: Germline encoded]]
+[[Category: Large Structures]]
-[[Category: Immune system]]
+[[Category: Staphylococcus aureus subsp. aureus MSSA476]]
-[[Category: Isdb]]
+[[Category: Deng X]]
-[[Category: Neat1]]

5d1z

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Current revision

IsdB NEAT1 bound by clone D4-10

Structural highlights

Function

Publication Abstract from PubMed

References

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