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5dlq
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5dlq is ON HOLD until Paper Publication Authors: Aksu, M., Trakhanov, S., Gorlich, D. Description: Crystal structure of RanGTP-Exportin 4-eIF5A com...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of RanGTP-Exportin 4-eIF5A complex== | |
| + | <StructureSection load='5dlq' size='340' side='right'caption='[[5dlq]], [[Resolution|resolution]] 3.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5dlq]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DLQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DLQ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CT:HYPUSINE'>5CT</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dlq OCA], [https://pdbe.org/5dlq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dlq RCSB], [https://www.ebi.ac.uk/pdbsum/5dlq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dlq ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/XPO4_MOUSE XPO4_MOUSE] Mediates the nuclear export of proteins (cargos) with broad substrate specificity. In the nucleus binds cooperatively to its cargo and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. XPO4 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Xpo4 is a bidirectional nuclear transport receptor that mediates nuclear export of eIF5A and Smad3 as well as import of Sox2 and SRY. How Xpo4 recognizes such a variety of cargoes is as yet unknown. Here we present the crystal structure of the RanGTP.Xpo4.eIF5A export complex at 3.2 A resolution. Xpo4 has a similar structure as CRM1, but the NES-binding site is occluded, and a new interaction site evolved that recognizes both globular domains of eIF5A. eIF5A contains hypusine, a unique amino acid with two positive charges, which is essential for cell viability and eIF5A function in translation. The hypusine docks into a deep, acidic pocket of Xpo4 and is thus a critical element of eIF5A's complex export signature. This further suggests that Xpo4 recognizes other cargoes differently, and illustrates how Xpo4 suppresses - in a chaperone-like manner - undesired interactions of eIF5A inside nuclei. | ||
| - | + | Structure of the exportin Xpo4 in complex with RanGTP and the hypusine-containing translation factor eIF5A.,Aksu M, Trakhanov S, Gorlich D Nat Commun. 2016 Jun 16;7:11952. doi: 10.1038/ncomms11952. PMID:27306458<ref>PMID:27306458</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Aksu | + | <div class="pdbe-citations 5dlq" style="background-color:#fffaf0;"></div> |
| - | [[Category: Gorlich | + | |
| - | [[Category: Trakhanov | + | ==See Also== |
| + | *[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]] | ||
| + | *[[Exportin 3D structures|Exportin 3D structures]] | ||
| + | *[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Aksu M]] | ||
| + | [[Category: Gorlich D]] | ||
| + | [[Category: Trakhanov S]] | ||
Current revision
Crystal structure of RanGTP-Exportin 4-eIF5A complex
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