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| | <StructureSection load='5dm9' size='340' side='right'caption='[[5dm9]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='5dm9' size='340' side='right'caption='[[5dm9]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5dm9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DM9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5DM9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5dm9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DM9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DM9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5dm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dm9 OCA], [http://pdbe.org/5dm9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5dm9 RCSB], [http://www.ebi.ac.uk/pdbsum/5dm9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5dm9 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dm9 OCA], [https://pdbe.org/5dm9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dm9 RCSB], [https://www.ebi.ac.uk/pdbsum/5dm9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dm9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | + | [https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Gallus gallus]] | | [[Category: Gallus gallus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lysozyme]]
| + | [[Category: Kobayashi J]] |
| - | [[Category: Kobayashi, J]] | + | [[Category: Nango E]] |
| - | [[Category: Nango, E]] | + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Serial femtosecond crystallography]]
| + | |
| Structural highlights
Function
LYSC_CHICK Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.[1]
Publication Abstract from PubMed
A liquid-droplet injector has been developed that delivers pristine microcrystals to an X-ray irradiation area for conducting serial femtosecond crystallography (SFX) with an X-ray free-electron laser (XFEL). By finely tuning the pulsed liquid droplets in time and space, a high hit rate of the XFEL pulses to microcrystals in the droplets was achieved for measurements using 5 microm tetragonal lysozyme crystals, which produced 4265 indexable diffraction images in about 30 min. The structure was determined at a resolution of 2.3 A from <0.3 mg of protein. With further improvements such as reduction of the droplet size, liquid droplets have considerable potential as a crystal carrier for SFX with low sample consumption.
Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography.,Mafune F, Miyajima K, Tono K, Takeda Y, Kohno JY, Miyauchi N, Kobayashi J, Joti Y, Nango E, Iwata S, Yabashi M Acta Crystallogr D Struct Biol. 2016 Apr;72(Pt 4):520-3. doi:, 10.1107/S2059798316001480. Epub 2016 Mar 24. PMID:27050131[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Maehashi K, Matano M, Irisawa T, Uchino M, Kashiwagi Y, Watanabe T. Molecular characterization of goose- and chicken-type lysozymes in emu (Dromaius novaehollandiae): evidence for extremely low lysozyme levels in emu egg white. Gene. 2012 Jan 15;492(1):244-9. doi: 10.1016/j.gene.2011.10.021. Epub 2011 Oct, 25. PMID:22044478 doi:10.1016/j.gene.2011.10.021
- ↑ Mafune F, Miyajima K, Tono K, Takeda Y, Kohno JY, Miyauchi N, Kobayashi J, Joti Y, Nango E, Iwata S, Yabashi M. Microcrystal delivery by pulsed liquid droplet for serial femtosecond crystallography. Acta Crystallogr D Struct Biol. 2016 Apr;72(Pt 4):520-3. doi:, 10.1107/S2059798316001480. Epub 2016 Mar 24. PMID:27050131 doi:http://dx.doi.org/10.1107/S2059798316001480
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