1u9f

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(New page: 200px<br /><applet load="1u9f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u9f, resolution 2.20&Aring;" /> '''Heterocyclic Peptide...)
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[[Image:1u9f.jpg|left|200px]]<br /><applet load="1u9f" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1u9f, resolution 2.20&Aring;" />
 
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'''Heterocyclic Peptide Backbone Modification in GCN4-pLI Based Coiled Coils: Replacement of K(15)L(16)'''<br />
 
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==Overview==
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==Heterocyclic Peptide Backbone Modification in GCN4-pLI Based Coiled Coils: Replacement of K(15)L(16)==
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In this paper, we present 1,2,3-triazole 2-amino acids incorporated as a dipeptide surrogate at three positions in the sequence of a known -helical coiled coil. Biophysical characterization indicates that the modified peptides retain much of the helical structure of the parent sequence, and that the thermodynamic stability of the coiled coil depends on the position of the incorporation of the -residue. Crystal structures obtained for each peptide give insight into the chemical behavior and conformational preferences of the non-natural amino acid and show that the triazole ring can participate in the backbone hydrogen bonding of the -helix as well as template an interhelical crossing between chains in the bundle.
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<StructureSection load='1u9f' size='340' side='right'caption='[[1u9f]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1u9f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U9F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U9F FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=TA4:(S)-2-[4-(AMINOMETHYL)-1H-1,2,3-TRIAZOL-1-YL]-4-METHYLPENTANOIC+ACID'>TA4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u9f OCA], [https://pdbe.org/1u9f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u9f RCSB], [https://www.ebi.ac.uk/pdbsum/1u9f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u9f ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In this paper, we present 1,2,3-triazole epsilon2-amino acids incorporated as a dipeptide surrogate at three positions in the sequence of a known alpha-helical coiled coil. Biophysical characterization indicates that the modified peptides retain much of the helical structure of the parent sequence, and that the thermodynamic stability of the coiled coil depends on the position of the incorporation of the epsilon-residue. Crystal structures obtained for each peptide give insight into the chemical behavior and conformational preferences of the non-natural amino acid and show that the triazole ring can participate in the backbone hydrogen bonding of the alpha-helix as well as template an interhelical crossing between chains in the bundle.
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==About this Structure==
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Heterocyclic peptide backbone modifications in an alpha-helical coiled coil.,Horne WS, Yadav MK, Stout CD, Ghadiri MR J Am Chem Soc. 2004 Dec 1;126(47):15366-7. PMID:15563148<ref>PMID:15563148</ref>
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1U9F is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with ACE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U9F OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Heterocyclic peptide backbone modifications in an alpha-helical coiled coil., Horne WS, Yadav MK, Stout CD, Ghadiri MR, J Am Chem Soc. 2004 Dec 1;126(47):15366-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15563148 15563148]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1u9f" style="background-color:#fffaf0;"></div>
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[[Category: Ghadiri, M.R.]]
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[[Category: Horne, W.S.]]
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[[Category: Stout, C.D.]]
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[[Category: Yadav, M.K.]]
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[[Category: ACE]]
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[[Category: heterocycic backbone modification]]
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[[Category: tetrameric alpha-helical coiled coil]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 03:33:22 2007''
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==See Also==
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*[[Gcn4 3D Structures|Gcn4 3D Structures]]
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*[[Gnc4 3D Structures|Gnc4 3D Structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Ghadiri MR]]
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[[Category: Horne WS]]
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[[Category: Stout CD]]
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[[Category: Yadav MK]]

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Heterocyclic Peptide Backbone Modification in GCN4-pLI Based Coiled Coils: Replacement of K(15)L(16)

PDB ID 1u9f

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