This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5edj
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5edj is ON HOLD until Paper Publication Authors: Sviridova, E., Bumba, L., Rezacova, P., Sebo, P., Kuta Smatanova, I. Description: Crystal structur...) |
|||
| (3 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the Neisseria meningitidis iron-regulated outer membrane lipoprotein FrpD== | |
| + | <StructureSection load='5edj' size='340' side='right'caption='[[5edj]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5edj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_meningitidis_MC58 Neisseria meningitidis MC58]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EDJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EDJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5edj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5edj OCA], [https://pdbe.org/5edj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5edj RCSB], [https://www.ebi.ac.uk/pdbsum/5edj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5edj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9K129_NEIMB Q9K129_NEIMB] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The iron-regulated protein FrpD from Neisseria meningitidis is an outer membrane lipoprotein that interacts with very high affinity (Kd ~ 0.2 nM) with the N-terminal domain of FrpC, a Type I-secreted protein from the Repeat in ToXin (RTX) protein family. In the presence of Ca2+, FrpC undergoes Ca2+ -dependent protein trans-splicing that includes an autocatalytic cleavage of the Asp414-Pro415 peptide bond and formation of an Asp414-Lys isopeptide bond. Here, we report the high-resolution structure of FrpD and describe the structure-function relationships underlying the interaction between FrpD and FrpC1-414. We identified FrpD residues involved in FrpC1-414 binding, which enabled localization of FrpD within the low-resolution SAXS model of the FrpD-FrpC1-414 complex. Moreover, the trans-splicing activity of FrpC resulted in covalent linkage of the FrpC1-414 fragment to plasma membrane proteins of epithelial cells in vitro, suggesting that formation of the FrpD-FrpC1-414 complex may be involved in the interaction of meningococci with the host cell surface. | ||
| - | + | Structural basis of the interaction between the putative adhesion-involved and iron-regulated FrpD and FrpC proteins of Neisseria meningitidis.,Sviridova E, Rezacova P, Bondar A, Veverka V, Novak P, Schenk G, Svergun DI, Kuta Smatanova I, Bumba L Sci Rep. 2017 Jan 13;7:40408. doi: 10.1038/srep40408. PMID:28084396<ref>PMID:28084396</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Bumba | + | <div class="pdbe-citations 5edj" style="background-color:#fffaf0;"></div> |
| - | [[Category: Kuta Smatanova | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: Sviridova | + | </StructureSection> |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Neisseria meningitidis MC58]] | ||
| + | [[Category: Bumba L]] | ||
| + | [[Category: Kuta Smatanova I]] | ||
| + | [[Category: Rezacova P]] | ||
| + | [[Category: Sebo P]] | ||
| + | [[Category: Sviridova E]] | ||
Current revision
Crystal structure of the Neisseria meningitidis iron-regulated outer membrane lipoprotein FrpD
| |||||||||||
