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5eza
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 5eza is ON HOLD Authors: Burton, A.J., Brady, R.L., Woolfson, D.N. Description: A de novo designed heptameric coiled coil CC-Hept-I18C-L22H [[Categ...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==A de novo designed heptameric coiled coil CC-Hept-I18C-L22H== | |
| + | <StructureSection load='5eza' size='340' side='right'caption='[[5eza]], [[Resolution|resolution]] 1.76Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5eza]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5EZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5EZA FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5eza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5eza OCA], [https://pdbe.org/5eza PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5eza RCSB], [https://www.ebi.ac.uk/pdbsum/5eza PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5eza ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The design of enzyme-like catalysts tests our understanding of sequence-to-structure/function relationships in proteins. Here we install hydrolytic activity predictably into a completely de novo and thermostable alpha-helical barrel, which comprises seven helices arranged around an accessible channel. We show that the lumen of the barrel accepts 21 mutations to functional polar residues. The resulting variant, which has cysteine-histidine-glutamic acid triads on each helix, hydrolyses p-nitrophenyl acetate with catalytic efficiencies that match the most-efficient redesigned hydrolases based on natural protein scaffolds. This is the first report of a functional catalytic triad engineered into a de novo protein framework. The flexibility of our system also allows the facile incorporation of unnatural side chains to improve activity and probe the catalytic mechanism. Such a predictable and robust construction of truly de novo biocatalysts holds promise for applications in chemical and biochemical synthesis. | ||
| - | + | Installing hydrolytic activity into a completely de novo protein framework.,Burton AJ, Thomson AR, Dawson WM, Brady RL, Woolfson DN Nat Chem. 2016 Sep;8(9):837-44. doi: 10.1038/nchem.2555. Epub 2016 Jul 4. PMID:27554410<ref>PMID:27554410</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 5eza" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Brady RL]] | ||
| + | [[Category: Burton AJ]] | ||
| + | [[Category: Woolfson DN]] | ||
Current revision
A de novo designed heptameric coiled coil CC-Hept-I18C-L22H
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