This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5f11

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:39, 12 July 2023) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==Crystal structure of Fructokinase from Vibrio cholerae O395 in fructose bound form==
==Crystal structure of Fructokinase from Vibrio cholerae O395 in fructose bound form==
-
<StructureSection load='5f11' size='340' side='right' caption='[[5f11]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+
<StructureSection load='5f11' size='340' side='right'caption='[[5f11]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[5f11]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F11 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F11 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[5f11]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O395 Vibrio cholerae O395]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F11 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F11 FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5f0z|5f0z]], [[5eyn|5eyn]], [[5ey7|5ey7]]</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructokinase Fructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.4 2.7.1.4] </span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f11 OCA], [https://pdbe.org/5f11 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f11 RCSB], [https://www.ebi.ac.uk/pdbsum/5f11 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f11 ProSAT]</span></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f11 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f11 OCA], [http://pdbe.org/5f11 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f11 RCSB], [http://www.ebi.ac.uk/pdbsum/5f11 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f11 ProSAT]</span></td></tr>
+
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/A0A0H3AER7_VIBC3 A0A0H3AER7_VIBC3]
-
Fructokinase (FK), one of the crucial enzymes for sugar metabolism in bacterial systems, catalyses the unidirectional phosphorylation reaction from fructose to fructose 6-phosphate, thereby allowing parallel entry of fructose into glycolysis beside glucose. The cscK gene from Vibrio cholerae O395 coding for the enzyme FK has been cloned, overexpressed in Escherichia coli BL21 (DE3) and purified using Ni-NTA affinity chromatography. Crystals of V. cholerae FK (Vc-FK) and its cocrystal with fructose, adenosine diphosphate (ADP) and Mg2+ were grown in the presence of polyethylene glycol 6000 and diffracted to 2.45 and 1.75 A resolution, respectively. Analysis of the diffraction data showed that both crystal forms have symmetry consistent with space group P2(1)2(1)2, but with different unit-cell parameters. Assuming the presence of two molecules in the asymmetric unit, the Matthews coefficient for the apo Vc-FK crystals was estimated to be 2.4 A3 Da(-1), which corresponds to a solvent content of 48%. The corresponding values for the ADP- and sugar-bound Vc-FK crystals were 2.1 A3 Da(-1) and 40%, respectively, assuming the presence of one molecule in the asymmetric unit.
+
-
Cloning, expression, purification, crystallization and preliminary X-ray analysis of a fructokinase from Vibrio cholerae O395.,Paul R, Nath S, Sen U Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1564-7., doi: 10.1107/S1744309112047598. Epub 2012 Nov 28. PMID:23192049<ref>PMID:23192049</ref>
+
==See Also==
-
 
+
*[[Fructokinase|Fructokinase]]
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 5f11" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Fructokinase]]
+
[[Category: Large Structures]]
-
[[Category: Nath, S]]
+
[[Category: Vibrio cholerae O395]]
-
[[Category: Paul, R]]
+
[[Category: Nath S]]
-
[[Category: Sen, U]]
+
[[Category: Paul R]]
-
[[Category: Fructose]]
+
[[Category: Sen U]]
-
[[Category: Kinase]]
+
-
[[Category: Sodium]]
+
-
[[Category: Transferase]]
+

Current revision

Crystal structure of Fructokinase from Vibrio cholerae O395 in fructose bound form

PDB ID 5f11

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5f11"
Personal tools