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8hnv
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==CryoEM structure of HpaCas9-sgRNA-dsDNA in the presence of AcrIIC4== | |
| - | + | <StructureSection load='8hnv' size='340' side='right'caption='[[8hnv]], [[Resolution|resolution]] 3.10Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[8hnv]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_parainfluenzae Haemophilus parainfluenzae] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HNV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HNV FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> | |
| - | [[Category: | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hnv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hnv OCA], [https://pdbe.org/8hnv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hnv RCSB], [https://www.ebi.ac.uk/pdbsum/8hnv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hnv ProSAT]</span></td></tr> |
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/F0ET08_HAEPA F0ET08_HAEPA] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.[HAMAP-Rule:MF_01480] | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Haemophilus parainfluenzae]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Cheng Z]] | ||
| + | [[Category: Sun W]] | ||
| + | [[Category: Wang J]] | ||
| + | [[Category: Wang Y]] | ||
| + | [[Category: Yang X]] | ||
Current revision
CryoEM structure of HpaCas9-sgRNA-dsDNA in the presence of AcrIIC4
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