1llm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1llm.jpg|left|200px]]
[[Image:1llm.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1llm |SIZE=350|CAPTION= <scene name='initialview01'>1llm</scene>, resolution 1.50&Aring;
+
The line below this paragraph, containing "STRUCTURE_1llm", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1llm| PDB=1llm | SCENE= }}
-
|RELATEDENTRY=[[1aay|1AAY]], [[1zaa|1ZAA]], [[1ysa|1YSA]], [[1g2d|1G2D]], [[1g2f|1G2F]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1llm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1llm OCA], [http://www.ebi.ac.uk/pdbsum/1llm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1llm RCSB]</span>
+
-
}}
+
'''Crystal Structure of a Zif23-GCN4 Chimera Bound to DNA'''
'''Crystal Structure of a Zif23-GCN4 Chimera Bound to DNA'''
Line 28: Line 25:
[[Category: Pabo, C O.]]
[[Category: Pabo, C O.]]
[[Category: Wolfe, S A.]]
[[Category: Wolfe, S A.]]
-
[[Category: dimerization]]
+
[[Category: Dimerization]]
-
[[Category: dna recognition]]
+
[[Category: Dna recognition]]
-
[[Category: leucine zipper]]
+
[[Category: Leucine zipper]]
-
[[Category: structure-based design]]
+
[[Category: Structure-based design]]
-
[[Category: x-ray crystallography]]
+
[[Category: X-ray crystallography]]
-
[[Category: zinc finger]]
+
[[Category: Zinc finger]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:02:20 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:04:17 2008''
+

Revision as of 21:02, 2 May 2008

Image:1llm.jpg

Template:STRUCTURE 1llm

Crystal Structure of a Zif23-GCN4 Chimera Bound to DNA


Overview

Proteins that employ dimerization domains to bind cooperatively to DNA have a number of potential advantages over monomers with regards to gene regulation. Using a combination of structure-based design and phage display, a dimeric Cys(2)His(2) zinc finger protein has been created that binds cooperatively to DNA via an attached leucine zipper dimerization domain. This chimera, derived from components of Zif268 and GCN4, displayed excellent DNA-binding specificity, and we now report the 1.5 A resolution cocrystal structure of the Zif268-GCN4 homodimer bound to DNA. This structure shows how phage display has annealed the DNA binding and dimerization domains into a single functional unit. Moreover, this chimera provides a potential platform for the creation heterodimeric zinc finger proteins that can regulate a desired target gene through cooperative DNA recognition.

About this Structure

1LLM is a Single protein structure of sequence from Mus musculus and saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure of a designed dimeric zinc finger protein bound to DNA., Wolfe SA, Grant RA, Pabo CO, Biochemistry. 2003 Nov 25;42(46):13401-9. PMID:14621985 Page seeded by OCA on Sat May 3 00:02:20 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1llm"
Personal tools