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3ly8

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Crystal structure of mutant D471E of the periplasmic domain of CadC

Structural highlights

3ly8 is a 1 chain structure with sequence from Escherichia coli str. K-12 substr. MG1655. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.9Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CADC_ECOLI Required for Pcad induction, a promoter upstream of cadBA that is responsible for the pH-regulated expression of CadA and CadB. Probably acts as an activating transcription factor.^[1]

Publication Abstract from PubMed

The membrane-integral transcriptional activator CadC comprises sensory and transcriptional regulatory functions within one polypeptide chain. Its C-terminal periplasmic domain, CadC(pd) , is responsible for sensing of environmental pH as well as for binding of the feedback inhibitor cadaverine. Here we describe the crystal structure of CadC(pd) (residues 188-512) solved at a resolution of 1.8 A via multiple wavelength anomalous dispersion (MAD) using a ReCl(6) (2-) derivative. CadC(pd) reveals a novel fold comprising two subdomains: an N-terminal subdomain dominated by a $\tilde \beta$sheet in contact with three alpha-helices and a C-terminal subdomain formed by a ten-membered alpha-helical bundle, which is oriented almost perpendicular to the helices in the first subdomain. Further to the native protein, crystal structures were also solved for its variants D471N and D471E, which show functionally different behavior in pH sensing. Interestingly, in the heavy metal derivative of CadC(pd) used for MAD phasing a ReCl(6) (2-) ion was found in a cavity located between the two subdomains. Amino acid side chains that coordinate this complex ion are conserved in CadC homologues from various bacterial species, suggesting a function of the cavity in the binding of cadaverine, which was supported by docking studies. Notably, CadC(pd) and its variants form dimers in solution, which can be explained by an extended, albeit rather polar interface between two symmetry-related monomers in the crystal structure. The occurrence of several acidic residues in this region suggests protonation-dependent changes in the mode of dimerization, which could eventually trigger transcriptional activation by CadC in the bacterial cytoplasm.

Crystal structure of the sensory domain of Escherichia coli CadC, a member of the ToxR-like protein family.,Eichinger A, Haneburger I, Koller C, Jung K, Skerra A Protein Sci. 2011 Jan 24. doi: 10.1002/pro.594. PMID:21308846^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Watson N, Dunyak DS, Rosey EL, Slonczewski JL, Olson ER. Identification of elements involved in transcriptional regulation of the Escherichia coli cad operon by external pH. J Bacteriol. 1992 Jan;174(2):530-40. PMID:1370290
↑ Eichinger A, Haneburger I, Koller C, Jung K, Skerra A. Crystal structure of the sensory domain of Escherichia coli CadC, a member of the ToxR-like protein family. Protein Sci. 2011 Jan 24. doi: 10.1002/pro.594. PMID:21308846 doi:10.1002/pro.594

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ly8"

Categories: Escherichia coli str. K-12 substr. MG1655 | Large Structures | Eichinger A | Skerra A

@@ Line 1: / Line 1: @@
 ==Crystal structure of mutant D471E of the periplasmic domain of CadC==
-<StructureSection load='3ly8' size='340' side='right' caption='[[3ly8]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
+<StructureSection load='3ly8' size='340' side='right'caption='[[3ly8]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ly8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LY8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LY8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ly8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_str._K-12_substr._MG1655 Escherichia coli str. K-12 substr. MG1655]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LY8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LY8 FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3lya|3lya]], [[3ly7|3ly7]], [[3ly9|3ly9]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b4133, cadC, JW4094 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ly8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ly8 OCA], [https://pdbe.org/3ly8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ly8 RCSB], [https://www.ebi.ac.uk/pdbsum/3ly8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ly8 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ly8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ly8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ly8 RCSB], [http://www.ebi.ac.uk/pdbsum/3ly8 PDBsum]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/CADC_ECOLI CADC_ECOLI] Required for Pcad induction, a promoter upstream of cadBA that is responsible for the pH-regulated expression of CadA and CadB. Probably acts as an activating transcription factor.<ref>PMID:1370290</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 15: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3ly8" style="background-color:#fffaf0;"></div>
 ==See Also==
-*[[Transcriptional activator|Transcriptional activator]]
+*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Escherichia coli]]
+[[Category: Escherichia coli str. K-12 substr. MG1655]]
-[[Category: Eichinger, A]]
+[[Category: Large Structures]]
-[[Category: Skerra, A]]
+[[Category: Eichinger A]]
-[[Category: Activator]]
+[[Category: Skerra A]]
-[[Category: Alpha domain]]
-[[Category: Alpha/beta domain]]
-[[Category: Dna-binding]]
-[[Category: Membrane]]
-[[Category: Signaling protein]]
-[[Category: Transcription regulation]]
-[[Category: Transmembrane]]
-[[Category: Two-component regulatory system]]

3ly8

From Proteopedia

Current revision

Crystal structure of mutant D471E of the periplasmic domain of CadC

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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