1llu

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[[Image:1llu.gif|left|200px]]
[[Image:1llu.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1llu |SIZE=350|CAPTION= <scene name='initialview01'>1llu</scene>, resolution 2.30&Aring;
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The line below this paragraph, containing "STRUCTURE_1llu", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= adh ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
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-->
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|DOMAIN=
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{{STRUCTURE_1llu| PDB=1llu | SCENE= }}
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|RELATEDENTRY=[[1ykf|1YKF]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1llu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1llu OCA], [http://www.ebi.ac.uk/pdbsum/1llu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1llu RCSB]</span>
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}}
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'''THE TERNARY COMPLEX OF PSEUDOMONAS AERUGINOSA ALCOHOL DEHYDROGENASE WITH ITS COENZYME AND WEAK SUBSTRATE'''
'''THE TERNARY COMPLEX OF PSEUDOMONAS AERUGINOSA ALCOHOL DEHYDROGENASE WITH ITS COENZYME AND WEAK SUBSTRATE'''
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[[Category: Meiri, G.]]
[[Category: Meiri, G.]]
[[Category: Peretz, M.]]
[[Category: Peretz, M.]]
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[[Category: alcohol dehydrogenase]]
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[[Category: Alcohol dehydrogenase]]
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[[Category: enzyme-coenzyme-substrate complex]]
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[[Category: Enzyme-coenzyme-substrate complex]]
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[[Category: nadh]]
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[[Category: Nadh]]
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[[Category: proton relay system]]
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[[Category: Proton relay system]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 00:02:47 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:04:21 2008''
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Revision as of 21:02, 2 May 2008

Image:1llu.gif

Template:STRUCTURE 1llu

THE TERNARY COMPLEX OF PSEUDOMONAS AERUGINOSA ALCOHOL DEHYDROGENASE WITH ITS COENZYME AND WEAK SUBSTRATE


Overview

Pseudomonas aeruginosa alcohol dehydrogenase (PaADH; ADH, EC 1.1.1.1) catalyzes the reversible oxidation of primary and secondary alcohols to the corresponding aldehydes and ketones, using NAD as coenzyme. We crystallized the ternary complex of PaADH with its coenzyme and a substrate molecule and determined its structure at a resolution of 2.3 A, using the molecular replacement method. The PaADH tetramer comprises four identical chains of 342 amino acid residues each and obeys ~222-point symmetry. The PaADH monomer is structurally similar to alcohol dehydrogenase monomers from vertebrates, archaea, and bacteria. The stabilization of the ternary complex of PaADH, the coenzyme, and the poor substrate ethylene glycol (k(cat) = 4.5 sec(-1); Km > 200 mM) was due to the blocked exit of the coenzyme in the crystalline state, combined with a high (2.5 M) concentration of the substrate. The structure of the ternary complex presents the precise geometry of the Zn coordination complex, the proton-shuttling system, and the hydride transfer path. The ternary complex structure also suggests that the low efficiency of ethylene glycol as a substrate results from the presence of a second hydroxyl group in this molecule.

About this Structure

1LLU is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

The ternary complex of Pseudomonas aeruginosa alcohol dehydrogenase with NADH and ethylene glycol., Levin I, Meiri G, Peretz M, Burstein Y, Frolow F, Protein Sci. 2004 Jun;13(6):1547-56. PMID:15152088 Page seeded by OCA on Sat May 3 00:02:47 2008

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