This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


5fja

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: '''Unreleased structure''' The entry 5fja is ON HOLD until sometime in the future Authors: Hoffmann, N.A., Jakobi, A.J., Moreno-Morcillo, M., Glatt, S., Kosinski, J., Hagen, W.J., Sachs...)
Current revision (06:53, 19 July 2023) (edit) (undo)
 
(7 intermediate revisions not shown.)
Line 1: Line 1:
-
'''Unreleased structure'''
 
-
The entry 5fja is ON HOLD until sometime in the future
+
==Cryo-EM structure of yeast RNA polymerase III at 4.7 A==
 +
<SX load='5fja' size='340' side='right' viewer='molstar' caption='[[5fja]], [[Resolution|resolution]] 4.65&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[5fja]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FJA FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.65&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fja OCA], [https://pdbe.org/5fja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fja RCSB], [https://www.ebi.ac.uk/pdbsum/5fja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fja ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RPC8_YEAST RPC8_YEAST] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNA. The RPC25/RPC8-RPC17/RPC9 subcomplex may bind Pol III transcripts emerging from the adjacent exit pore during elongation.
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Transcription of genes encoding small structured RNAs such as transfer RNAs, spliceosomal U6 small nuclear RNA and ribosomal 5S RNA is carried out by RNA polymerase III (Pol III), the largest yet structurally least characterized eukaryotic RNA polymerase. Here we present the cryo-electron microscopy structures of the Saccharomyces cerevisiae Pol III elongating complex at 3.9 A resolution and the apo Pol III enzyme in two different conformations at 4.6 and 4.7 A resolution, respectively, which allow the building of a 17-subunit atomic model of Pol III. The reconstructions reveal the precise orientation of the C82-C34-C31 heterotrimer in close proximity to the stalk. The C53-C37 heterodimer positions residues involved in transcription termination close to the non-template DNA strand. In the apo Pol III structures, the stalk adopts different orientations coupled with closed and open conformations of the clamp. Our results provide novel insights into Pol III-specific transcription and the adaptation of Pol III towards its small transcriptional targets.
-
Authors: Hoffmann, N.A., Jakobi, A.J., Moreno-Morcillo, M., Glatt, S., Kosinski, J., Hagen, W.J., Sachse, C., Muller, C.W.
+
Molecular structures of unbound and transcribing RNA polymerase III.,Hoffmann NA, Jakobi AJ, Moreno-Morcillo M, Glatt S, Kosinski J, Hagen WJ, Sachse C, Muller CW Nature. 2015 Nov 25. doi: 10.1038/nature16143. PMID:26605533<ref>PMID:26605533</ref>
-
Description: Cryo-EM structure of yeast RNA polymerase III at 4.7 A
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
[[Category: Unreleased Structures]]
+
</div>
-
[[Category: Kosinski, J]]
+
<div class="pdbe-citations 5fja" style="background-color:#fffaf0;"></div>
-
[[Category: Muller, C.W]]
+
 
-
[[Category: Hagen, W.J]]
+
==See Also==
-
[[Category: Hoffmann, N.A]]
+
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
-
[[Category: Glatt, S]]
+
== References ==
-
[[Category: Jakobi, A.J]]
+
<references/>
-
[[Category: Sachse, C]]
+
__TOC__
-
[[Category: Moreno-Morcillo, M]]
+
</SX>
 +
[[Category: Large Structures]]
 +
[[Category: Saccharomyces cerevisiae]]
 +
[[Category: Glatt S]]
 +
[[Category: Hagen WJ]]
 +
[[Category: Hoffmann NA]]
 +
[[Category: Jakobi AJ]]
 +
[[Category: Kosinski J]]
 +
[[Category: Moreno-Morcillo M]]
 +
[[Category: Muller CW]]
 +
[[Category: Sachse C]]

Current revision

Cryo-EM structure of yeast RNA polymerase III at 4.7 A

5fja, resolution 4.65Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5fja"
Personal tools