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8fvh
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Pseudomonas phage E217 neck (portal, head-to-tail connector, collar and gateway proteins)== | |
| + | <StructureSection load='8fvh' size='340' side='right'caption='[[8fvh]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8fvh]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_phage_vB_PaeM_E217 Pseudomonas phage vB_PaeM_E217]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8FVH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8FVH FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8fvh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8fvh OCA], [https://pdbe.org/8fvh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8fvh RCSB], [https://www.ebi.ac.uk/pdbsum/8fvh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8fvh ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A2K8I4A6_9CAUD A0A2K8I4A6_9CAUD] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | E217 is a Pseudomonas phage used in an experimental cocktail to eradicate cystic fibrosis-associated Pseudomonas aeruginosa. Here, we describe the structure of the whole E217 virion before and after DNA ejection at 3.1 A and 4.5 A resolution, respectively, determined using cryogenic electron microscopy (cryo-EM). We identify and build de novo structures for 19 unique E217 gene products, resolve the tail genome-ejection machine in both extended and contracted states, and decipher the complete architecture of the baseplate formed by 66 polypeptide chains. We also determine that E217 recognizes the host O-antigen as a receptor, and we resolve the N-terminal portion of the O-antigen-binding tail fiber. We propose that E217 design principles presented in this paper are conserved across PB1-like Myoviridae phages of the Pbunavirus genus that encode a ~1.4 MDa baseplate, dramatically smaller than the coliphage T4. | ||
| - | + | High-resolution cryo-EM structure of the Pseudomonas bacteriophage E217.,Li F, Hou CD, Lokareddy RK, Yang R, Forti F, Briani F, Cingolani G Nat Commun. 2023 Jul 8;14(1):4052. doi: 10.1038/s41467-023-39756-z. PMID:37422479<ref>PMID:37422479</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8fvh" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Hou | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pseudomonas phage vB_PaeM_E217]] | ||
| + | [[Category: Cingolani G]] | ||
| + | [[Category: Hou C]] | ||
| + | [[Category: Li F]] | ||
Current revision
Pseudomonas phage E217 neck (portal, head-to-tail connector, collar and gateway proteins)
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