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5fzt

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Revision as of 13:33, 26 July 2023

The crystal structure of R7R8 in complex with a DLC1 fragment.

Structural highlights

5fzt is a 2 chain structure with sequence from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.1Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TLN1_MOUSE Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.

Publication Abstract from PubMed

Cell migration requires coordination between integrin-mediated cell adhesion to the extracellular matrix and force applied to adhesion sites. Talin plays a key role in coupling integrin receptors to the actomyosin contractile machinery, while deleted in liver cancer 1 (DLC1) is a Rho GAP that binds talin and regulates Rho, and therefore actomyosin contractility. We show that the LD motif of DLC1 forms a helix that binds to the four-helix bundle of the talin R8 domain in a canonical triple-helix arrangement. We demonstrate that the same R8 surface interacts with the paxillin LD1 and LD2 motifs. We identify key charged residues that stabilize the R8 interactions with LD motifs and demonstrate their importance in vitro and in cells. Our results suggest a network of competitive interactions in adhesion complexes that involve LD motifs, and identify mutations that can be used to analyze the biological roles of specific protein-protein interactions in cell migration.

LD Motif Recognition by Talin: Structure of the Talin-DLC1 Complex.,Zacharchenko T, Qian X, Goult BT, Jethwa D, Almeida TB, Ballestrem C, Critchley DR, Lowy DR, Barsukov IL Structure. 2016 May 31. pii: S0969-2126(16)30077-6. doi:, 10.1016/j.str.2016.04.016. PMID:27265849^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zacharchenko T, Qian X, Goult BT, Jethwa D, Almeida TB, Ballestrem C, Critchley DR, Lowy DR, Barsukov IL. LD Motif Recognition by Talin: Structure of the Talin-DLC1 Complex. Structure. 2016 May 31. pii: S0969-2126(16)30077-6. doi:, 10.1016/j.str.2016.04.016. PMID:27265849 doi:http://dx.doi.org/10.1016/j.str.2016.04.016

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5fzt"

@@ Line 3: / Line 3: @@
 <StructureSection load='5fzt' size='340' side='right'caption='[[5fzt]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5fzt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FZT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5FZT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5fzt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FZT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FZT FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5fzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fzt OCA], [http://pdbe.org/5fzt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fzt RCSB], [http://www.ebi.ac.uk/pdbsum/5fzt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fzt ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fzt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fzt OCA], [https://pdbe.org/5fzt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fzt RCSB], [https://www.ebi.ac.uk/pdbsum/5fzt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fzt ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/TLN1_MOUSE TLN1_MOUSE]] Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. [[http://www.uniprot.org/uniprot/RHG07_HUMAN RHG07_HUMAN]] Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality.<ref>PMID:18786931</ref> <ref>PMID:19170769</ref> <ref>PMID:19710422</ref>
+[https://www.uniprot.org/uniprot/TLN1_MOUSE TLN1_MOUSE] Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 25: / Line 26: @@
 __TOC__
 </StructureSection>
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Almeida, T]]
+[[Category: Almeida T]]
-[[Category: Ballestrem, C]]
+[[Category: Ballestrem C]]
-[[Category: Barsukov, I L]]
+[[Category: Barsukov IL]]
-[[Category: Critchley, D R]]
+[[Category: Critchley DR]]
-[[Category: Goult, B T]]
+[[Category: Goult BT]]
-[[Category: Jethwa, D]]
+[[Category: Jethwa D]]
-[[Category: Lowy, D R]]
+[[Category: Lowy DR]]
-[[Category: Qian, X]]
+[[Category: Qian X]]
-[[Category: Zacharchenko, T]]
+[[Category: Zacharchenko T]]
-[[Category: Dlc1]]
-[[Category: Focal adhesion]]
-[[Category: Structural protein]]
-[[Category: Talin]]

5fzt

From Proteopedia

Revision as of 13:33, 26 July 2023

The crystal structure of R7R8 in complex with a DLC1 fragment.

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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