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17gs

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(New page: 200px<br /> <applet load="17gs" size="450" color="white" frame="true" align="right" spinBox="true" caption="17gs, resolution 1.90&Aring;" /> '''GLUTATHIONE S-TRANS...)
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[[Image:17gs.gif|left|200px]]<br />
 
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<applet load="17gs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="17gs, resolution 1.90&Aring;" />
 
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'''GLUTATHIONE S-TRANSFERASE P1-1'''<br />
 
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==Overview==
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==GLUTATHIONE S-TRANSFERASE P1-1==
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The human pi-class glutathione S-transferase (hGST P1-1) is a target for, structure-based inhibitor design with the aim of developing drugs that, could be used as adjuvants in chemotherapeutic treatment. Here we present, seven crystal structures of the enzyme in complex with substrate, (glutathione) and two inhibitors (S-hexyl glutathione and gamma-glutamyl-, (S-benzyl)cysteinyl-D-phenylglycine). The binding of the modified, glutathione inhibitor, gamma-glutamyl-(S-benzyl)cysteinyl-D-phenylglycine, has been characterized with the phenyl group stacking against the benzyl, moiety of the inhibitor and making interactions with the active-site, residues Phe8 and Trp38. The structure provides an explanation as to why, this compound inhibits the pi-class GST much better than the other GST, classes. The structure of the enzyme in complex with glutathione has been, determined to high resolution (1.9 to 2.2 A) in three different crystal, forms and at two different temperatures (100 and 288 K). In one crystal, form, the direct hydrogen-bonding interaction between the hydroxyl group, of Tyr7, a residue involved in catalysis, and the thiol group of the, substrate, glutathione, is broken and replaced by a water molecule that, mediates the interaction. The hydrogen-bonding partner of the hydroxyl, group of Tyr108, another residue implicated in the catalysis, is, space-group dependent. A high-resolution (2.0 A) structure of the enzyme, in complex with S-hexyl glutathione in a new crystal form is presented., The enzyme-inhibitor complexes show that the binding of ligand into the, electrophilic binding site does not lead to any conformational changes of, the protein.
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<StructureSection load='17gs' size='340' side='right'caption='[[17gs]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[17gs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=17GS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=17GS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTX:S-HEXYLGLUTATHIONE'>GTX</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=17gs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=17gs OCA], [https://pdbe.org/17gs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=17gs RCSB], [https://www.ebi.ac.uk/pdbsum/17gs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=17gs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GSTP1_HUMAN GSTP1_HUMAN] Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration.<ref>PMID:21668448</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/7g/17gs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=17gs ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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17GS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with GTX and MES as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=17GS OCA].
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*[[Glutathione S-transferase 3D structures|Glutathione S-transferase 3D structures]]
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== References ==
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==Reference==
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<references/>
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The structures of human glutathione transferase P1-1 in complex with glutathione and various inhibitors at high resolution., Oakley AJ, Lo Bello M, Battistoni A, Ricci G, Rossjohn J, Villar HO, Parker MW, J Mol Biol. 1997 Nov 21;274(1):84-100. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9398518 9398518]
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__TOC__
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[[Category: Glutathione transferase]]
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bello, M.Lo.]]
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[[Category: Lo Bello M]]
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[[Category: Oakley, A.J.]]
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[[Category: Oakley AJ]]
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[[Category: Parker, M.W.]]
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[[Category: Parker MW]]
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[[Category: GTX]]
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[[Category: MES]]
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[[Category: detoxification]]
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[[Category: glutathione transferase]]
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[[Category: k54a mutant]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 15:53:14 2007''
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Current revision

GLUTATHIONE S-TRANSFERASE P1-1

PDB ID 17gs

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