1a2b

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(New page: 200px<br /> <applet load="1a2b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1a2b, resolution 2.4&Aring;" /> '''HUMAN RHOA COMPLEXED...)
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[[Image:1a2b.gif|left|200px]]<br />
 
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<applet load="1a2b" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a2b, resolution 2.4&Aring;" />
 
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'''HUMAN RHOA COMPLEXED WITH GTP ANALOGUE'''<br />
 
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==Overview==
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==HUMAN RHOA COMPLEXED WITH GTP ANALOGUE==
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The 2.4-A resolution crystal structure of a dominantly active form of the, small guanosine triphosphatase (GTPase) RhoA, RhoAV14, complexed with the, nonhydrolyzable GTP analogue, guanosine 5'-3-O-(thio)triphosphate, (GTPgammaS), reveals a fold similar to RhoA-GDP, which has been recently, reported (Wei, Y., Zhang, Y., Derewenda, U., Liu, X., Minor, W., Nakamoto, R. K., Somlyo, A. V., Somlyo, A. P., and Derewenda, Z. S. (1997) Nat., Struct. Biol. 4, 699-703), but shows large conformational differences, localized in switch I and switch II. These changes produce hydrophobic, patches on the molecular surface of switch I, which has been suggested to, be involved in its effector binding. Compared with H-Ras and other GTPases, bound to GTP or GTP analogues, the significant conformational differences, are located in regions involving switches I and II and part of the, antiparallel beta-sheet between switches I and II. Key residues that, produce these conformational differences were identified. In addition to, these differences, RhoA contains four insertion or deletion sites with an, extra helical subdomain that seems to be characteristic of members of the, Rho family, including Rac1, but with several variations in details. These, sites also display large displacements from those of H-Ras. The, ADP-ribosylation residue, Asn41, by C3-like exoenzymes stacks on the, indole ring of Trp58 with a hydrogen bond to the main chain of Glu40. The, recognition of the guanosine moiety of GTPgammaS by the GTPase contains, water-mediated hydrogen bonds, which seem to be common in the Rho family., These structural differences provide an insight into specific interaction, sites with the effectors, as well as with modulators such as guanine, nucleotide exchange factor (GEF) and guanine nucleotide dissociation, inhibitor (GDI).
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<StructureSection load='1a2b' size='340' side='right'caption='[[1a2b]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1a2b]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A2B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A2B FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a2b OCA], [https://pdbe.org/1a2b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a2b RCSB], [https://www.ebi.ac.uk/pdbsum/1a2b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a2b ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RHOA_HUMAN RHOA_HUMAN] Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization.<ref>PMID:8910519</ref> <ref>PMID:9121475</ref> <ref>PMID:12900402</ref> <ref>PMID:16103226</ref> <ref>PMID:16236794</ref> <ref>PMID:19934221</ref> <ref>PMID:20937854</ref> <ref>PMID:20974804</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a2/1a2b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a2b ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The 2.4-A resolution crystal structure of a dominantly active form of the small guanosine triphosphatase (GTPase) RhoA, RhoAV14, complexed with the nonhydrolyzable GTP analogue, guanosine 5'-3-O-(thio)triphosphate (GTPgammaS), reveals a fold similar to RhoA-GDP, which has been recently reported (Wei, Y., Zhang, Y., Derewenda, U., Liu, X., Minor, W., Nakamoto, R. K., Somlyo, A. V., Somlyo, A. P., and Derewenda, Z. S. (1997) Nat. Struct. Biol. 4, 699-703), but shows large conformational differences localized in switch I and switch II. These changes produce hydrophobic patches on the molecular surface of switch I, which has been suggested to be involved in its effector binding. Compared with H-Ras and other GTPases bound to GTP or GTP analogues, the significant conformational differences are located in regions involving switches I and II and part of the antiparallel beta-sheet between switches I and II. Key residues that produce these conformational differences were identified. In addition to these differences, RhoA contains four insertion or deletion sites with an extra helical subdomain that seems to be characteristic of members of the Rho family, including Rac1, but with several variations in details. These sites also display large displacements from those of H-Ras. The ADP-ribosylation residue, Asn41, by C3-like exoenzymes stacks on the indole ring of Trp58 with a hydrogen bond to the main chain of Glu40. The recognition of the guanosine moiety of GTPgammaS by the GTPase contains water-mediated hydrogen bonds, which seem to be common in the Rho family. These structural differences provide an insight into specific interaction sites with the effectors, as well as with modulators such as guanine nucleotide exchange factor (GEF) and guanine nucleotide dissociation inhibitor (GDI).
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==About this Structure==
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Crystal structure of human RhoA in a dominantly active form complexed with a GTP analogue.,Ihara K, Muraguchi S, Kato M, Shimizu T, Shirakawa M, Kuroda S, Kaibuchi K, Hakoshima T J Biol Chem. 1998 Apr 17;273(16):9656-66. PMID:9545299<ref>PMID:9545299</ref>
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1A2B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG and GSP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A2B OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of human RhoA in a dominantly active form complexed with a GTP analogue., Ihara K, Muraguchi S, Kato M, Shimizu T, Shirakawa M, Kuroda S, Kaibuchi K, Hakoshima T, J Biol Chem. 1998 Apr 17;273(16):9656-66. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9545299 9545299]
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</div>
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[[Category: Homo sapiens]]
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<div class="pdbe-citations 1a2b" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Hakoshima, T.]]
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[[Category: Ihara, K.]]
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[[Category: Kaibuchi, K.]]
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[[Category: Kato, M.]]
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[[Category: Kuroda, S.]]
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[[Category: Muraguchi, S.]]
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[[Category: Shimizu, T.]]
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[[Category: Shirakawa, M.]]
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[[Category: GSP]]
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[[Category: MG]]
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[[Category: gtpase]]
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[[Category: oncogene protein]]
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[[Category: ras superfamily]]
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[[Category: signal transduction]]
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[[Category: small g-protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 15:54:45 2007''
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==See Also==
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*[[Rho GTPase 3D structures|Rho GTPase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
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[[Category: Hakoshima T]]
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[[Category: Ihara K]]
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[[Category: Kaibuchi K]]
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[[Category: Kato M]]
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[[Category: Kuroda S]]
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[[Category: Muraguchi S]]
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[[Category: Shimizu T]]
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[[Category: Shirakawa M]]

Current revision

HUMAN RHOA COMPLEXED WITH GTP ANALOGUE

PDB ID 1a2b

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