1bh3

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{{Seed}}
 
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[[Image:1bh3.png|left|200px]]
 
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==E1M, A116K MUTANT OF RH. BLASTICA PORIN==
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The line below this paragraph, containing "STRUCTURE_1bh3", creates the "Structure Box" on the page.
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<StructureSection load='1bh3' size='340' side='right'caption='[[1bh3]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1bh3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Fuscovulum_blasticum Fuscovulum blasticum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BH3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BH3 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene></td></tr>
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{{STRUCTURE_1bh3| PDB=1bh3 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bh3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bh3 OCA], [https://pdbe.org/1bh3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bh3 RCSB], [https://www.ebi.ac.uk/pdbsum/1bh3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bh3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PORI_FUSBL PORI_FUSBL] Forms channels that allow the passive diffusion of small hydrophilic solutes up to an exclusion limit of about 0.6 kDa.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bh/1bh3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bh3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The general diffusion porin from Rhodopseudomonas blastica was produced in large amounts in Escherichia coli inclusion bodies and (re)natured to the exact native structure. Here, we report on 13 mutants at the pore eyelet giving rise to new diffusion properties as measured in planar lipid bilayer experiments. The crystal structures of seven of these mutants were established. The effects of charge-modifying mutations at the pore eyelet are consistent with the known selectivity for cations. Deletions of 16 and 27 residues of the constriction loop L3 resulted in labile trimers and pores. The reduction of the eyelet cross section by introducing tryptophans gave rise to a closely correlated decrease of the conductivities. A mutant with six newly introduced tryptophans in the eyelet closed its pore in a defined manner within seconds under a voltage of 20 mV, suggesting the existence of two states. The results indicate that the pore can be engineered in a rational manner.
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===E1M, A116K MUTANT OF RH. BLASTICA PORIN===
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Porin mutants with new channel properties.,Schmid B, Maveyraud L, Kromer M, Schulz GE Protein Sci. 1998 Jul;7(7):1603-11. PMID:9684893<ref>PMID:9684893</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1bh3" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_9684893}}, adds the Publication Abstract to the page
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*[[Porin 3D structures|Porin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 9684893 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9684893}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Fuscovulum blasticum]]
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1BH3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_blasticus Rhodobacter blasticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BH3 OCA].
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[[Category: Large Structures]]
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[[Category: Maveyraud L]]
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==Reference==
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[[Category: Schmid B]]
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Porin mutants with new channel properties., Schmid B, Maveyraud L, Kromer M, Schulz GE, Protein Sci. 1998 Jul;7(7):1603-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9684893 9684893]
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[[Category: Schulz GE]]
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[[Category: Rhodobacter blasticus]]
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[[Category: Single protein]]
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[[Category: Maveyraud, L.]]
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[[Category: Schmid, B.]]
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[[Category: Schulz, G E.]]
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[[Category: Integral membrane protein]]
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[[Category: Pore eyelet mutant]]
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[[Category: Porin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 19:09:23 2008''
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Current revision

E1M, A116K MUTANT OF RH. BLASTICA PORIN

PDB ID 1bh3

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