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6msf
From Proteopedia
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| - | [[Image:6msf.gif|left|200px]]<br /><applet load="6msf" size="350" color="white" frame="true" align="right" spinBox="true" | ||
| - | caption="6msf, resolution 2.800Å" /> | ||
| - | '''F6 APTAMER MS2 COAT PROTEIN COMPLEX'''<br /> | ||
| - | == | + | ==F6 APTAMER MS2 COAT PROTEIN COMPLEX== |
| + | <StructureSection load='6msf' size='340' side='right'caption='[[6msf]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6msf]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_MS2 Escherichia phage MS2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MSF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MSF FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6msf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6msf OCA], [https://pdbe.org/6msf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6msf RCSB], [https://www.ebi.ac.uk/pdbsum/6msf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6msf ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CAPSD_BPMS2 CAPSD_BPMS2] Self-assembles to form the T=3 icosahedral virus shell that protects the viral nucleic acid. Acts as a translational repressor by binding with high specificity to a single stem-loop structure in the genomic RNA that contains the initiation codon of the gene for the viral replicase. Involved in virus assembly through the interaction between a capsid protein dimer and the multiple packaging signals present in the RNA genome.<ref>PMID:16531233</ref> <ref>PMID:18662904</ref> <ref>PMID:26608810</ref> <ref>PMID:8254664</ref> <ref>PMID:9245600</ref> <ref>PMID:9469847</ref> | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ms/6msf_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=6msf ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
The crystal structure, at 2.8 A resolution, of an RNA aptamer bound to bacteriophage MS2 coat protein has been determined. It provides an opportunity to compare the interactions of MS2 coat protein and wild type operator with those of an aptamer, whose secondary structure differs from the wild type RNA in having a three-base loop (compared to a tetraloop) and an additional base pair between this loop and the sequence-specific recognition element in the stem. The RNA binds in the same location on the coat protein as the wild type operator and maintains many of the same RNA-protein interactions. In order to achieve this, the RNA stem loop undergoes a concerted rearrangement of the 3' side while leaving the 5' side and the loop interactions largely unchanged, illustrating the ability of RNA to present similar molecular recognition surfaces from distinct primary and secondary structures. | The crystal structure, at 2.8 A resolution, of an RNA aptamer bound to bacteriophage MS2 coat protein has been determined. It provides an opportunity to compare the interactions of MS2 coat protein and wild type operator with those of an aptamer, whose secondary structure differs from the wild type RNA in having a three-base loop (compared to a tetraloop) and an additional base pair between this loop and the sequence-specific recognition element in the stem. The RNA binds in the same location on the coat protein as the wild type operator and maintains many of the same RNA-protein interactions. In order to achieve this, the RNA stem loop undergoes a concerted rearrangement of the 3' side while leaving the 5' side and the loop interactions largely unchanged, illustrating the ability of RNA to present similar molecular recognition surfaces from distinct primary and secondary structures. | ||
| - | + | Crystal structure of an RNA aptamer-protein complex at 2.8 A resolution.,Convery MA, Rowsell S, Stonehouse NJ, Ellington AD, Hirao I, Murray JB, Peabody DS, Phillips SE, Stockley PG Nat Struct Biol. 1998 Feb;5(2):133-9. PMID:9461079<ref>PMID:9461079</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[Category: | + | <div class="pdbe-citations 6msf" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Convery | + | <references/> |
| - | [[Category: Ellington | + | __TOC__ |
| - | [[Category: Hirao | + | </StructureSection> |
| - | [[Category: Murray | + | [[Category: Escherichia phage MS2]] |
| - | [[Category: Peabody | + | [[Category: Large Structures]] |
| - | [[Category: Phillips | + | [[Category: Convery MA]] |
| - | [[Category: Rowsell | + | [[Category: Ellington AD]] |
| - | [[Category: Stockley | + | [[Category: Hirao I]] |
| - | [[Category: Stonehouse | + | [[Category: Murray JB]] |
| - | + | [[Category: Peabody DS]] | |
| - | + | [[Category: Phillips SEV]] | |
| - | + | [[Category: Rowsell S]] | |
| - | + | [[Category: Stockley PG]] | |
| - | + | [[Category: Stonehouse NJ]] | |
| - | + | ||
| - | + | ||
Current revision
F6 APTAMER MS2 COAT PROTEIN COMPLEX
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