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1lvr

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IC3 of CB1 (L431A,A432L) Bound to G(alpha)i

Structural highlights

1lvr is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CNR1_HUMAN Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.^[1]

Publication Abstract from PubMed

The structure of the C-terminal region of the third cytoplasmic loop (IC3) of the cannabinoid receptor one (CB1) bound to G(alphai1) has been determined using transferred nuclear Overhauser effects (NOEs). The wild-type IC3 sequence is helical when associated with G(alphai1). In contrast, a peptide containing the amino-acid inversion, Ala(341)-Leu(342) adopts a single turn. These findings correlate with the attenuated G(i) association of CB1 with the Ala(341)-Leu(342) mutation previously observed in vivo and the diminished stimulation of G(alphai1) GTPase activity by the corresponding peptide demonstrated in vitro here. These results, the first to report the structure of a GPCR domain while associated with G protein, imply the C-terminus of CB1 IC3, a region with high-sequence conservation among G-protein coupled receptors, must be helical for efficient coupling and activation of the G(i) protein.

Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity.,Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF Protein Sci. 2002 Oct;11(10):2526-31. PMID:12237474^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ryberg E, Vu HK, Larsson N, Groblewski T, Hjorth S, Elebring T, Sjogren S, Greasley PJ. Identification and characterisation of a novel splice variant of the human CB1 receptor. FEBS Lett. 2005 Jan 3;579(1):259-64. PMID:15620723 doi:http://dx.doi.org/10.1016/j.febslet.2004.11.085
↑ Ulfers AL, McMurry JL, Miller A, Wang L, Kendall DA, Mierke DF. Cannabinoid receptor-G protein interactions: G(alphai1)-bound structures of IC3 and a mutant with altered G protein specificity. Protein Sci. 2002 Oct;11(10):2526-31. PMID:12237474

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1lvr"

@@ Line 1: / Line 1: @@
 ==IC3 of CB1 (L431A,A432L) Bound to G(alpha)i==
-<StructureSection load='1lvr' size='340' side='right'caption='[[1lvr]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
+<StructureSection load='1lvr' size='340' side='right'caption='[[1lvr]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1lvr]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LVR FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1lvr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LVR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LVR FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lvq|1lvq]]</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvr OCA], [http://pdbe.org/1lvr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lvr RCSB], [http://www.ebi.ac.uk/pdbsum/1lvr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lvr ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lvr OCA], [https://pdbe.org/1lvr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lvr RCSB], [https://www.ebi.ac.uk/pdbsum/1lvr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lvr ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CNR1_HUMAN CNR1_HUMAN]] Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.<ref>PMID:15620723</ref>
+[https://www.uniprot.org/uniprot/CNR1_HUMAN CNR1_HUMAN] Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding.<ref>PMID:15620723</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Kendall, D A]]
+[[Category: Kendall DA]]
-[[Category: McMurry, J L]]
+[[Category: McMurry JL]]
-[[Category: Mierke, D F]]
+[[Category: Mierke DF]]
-[[Category: Miller, A]]
+[[Category: Miller A]]
-[[Category: Ulfers, A L]]
+[[Category: Ulfers AL]]
-[[Category: Wang, L]]
+[[Category: Wang L]]
-[[Category: Alpha domain of g protein i]]
-[[Category: Signaling protein]]
-[[Category: Transferred no]]

1lvr

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Current revision

IC3 of CB1 (L431A,A432L) Bound to G(alpha)i

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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