This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

1pjd

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Structure and Topology of a Peptide Segment of the 6th Transmembrane Domain of the Saccharomyces cerevisiae alpha-Factor Receptor in Phospholipid Bilayers

Structural highlights

1pjd is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solid-state NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

STE2_YEAST Receptor for the peptide pheromone alpha factor, the mating factor of yeast.

Publication Abstract from PubMed

A detailed analysis of the structure of an 18-residue peptide AQSLLVPSIIFILAYSLK [M6(252-269, C252A)] in 1,2-dimyristoyl-sn-glycero-phosphocholine bilayers was carried out using solid state NMR and attenuated total reflection Fourier transform infrared spectroscopy. The peptide corresponds to a portion of the 6th transmembrane domain of the alpha-factor receptor of Saccharomyces cerevisiae. Ten homologs of M6(252-269, C252A) were synthesized in which individual residues were labeled with (15)N. One- and two-dimensional solid state NMR experiments were used to determine the chemical shifts and (1)H-(15)N dipolar coupling constants for the (15)N-labeled peptides in oriented dimyristoylphosphatidylcholine bilayers on stacked glass plates. These parameters were used to calculate the structure and orientation of M6(252-269, C252A) in the bilayers. The results indicate that the carboxyl terminal residues (9-14) are alpha-helical and oriented with an angle of about 8 degrees with respect to the bilayer normal. Independently, an attenuated total reflection Fourier transform infrared spectroscopy analysis on M6(252-269, C252A) in a 1,2-dimyristoyl-sn-glycero-phosphocholine bilayer concluded that the helix tilt angle was about 12.5 degrees. The results on the structure of M6(252-269, C252A) in bilayers are in good agreement with the structure determined in trifluoroethanol/water solutions (B. Arshava et al. Biopolymers, 1998, Vol. 46, pp. 343-357). The present study shows that solid state NMR spectroscopy can provide high resolution information on the structure of transmembrane domains of a G protein-coupled receptor.

Structure and topology of a peptide segment of the 6th transmembrane domain of the Saccharomyces cerevisae alpha-factor receptor in phospholipid bilayers.,Valentine KG, Liu SF, Marassi FM, Veglia G, Opella SJ, Ding FX, Wang SH, Arshava B, Becker JM, Naider F Biopolymers. 2001 Oct 5;59(4):243-56. PMID:11473349^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Valentine KG, Liu SF, Marassi FM, Veglia G, Opella SJ, Ding FX, Wang SH, Arshava B, Becker JM, Naider F. Structure and topology of a peptide segment of the 6th transmembrane domain of the Saccharomyces cerevisae alpha-factor receptor in phospholipid bilayers. Biopolymers. 2001 Oct 5;59(4):243-56. PMID:11473349 doi:<243::AID-BIP1021>3.0.CO;2-H http://dx.doi.org/10.1002/1097-0282(20011005)59:4<243::AID-BIP1021>3.0.CO;2-H

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1pjd"

@@ Line 1: / Line 1: @@
 ==Structure and Topology of a Peptide Segment of the 6th Transmembrane Domain of the Saccharomyces cerevisiae alpha-Factor Receptor in Phospholipid Bilayers==
-<StructureSection load='1pjd' size='340' side='right'caption='[[1pjd]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
+<StructureSection load='1pjd' size='340' side='right'caption='[[1pjd]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1pjd]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PJD FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1pjd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PJD FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pjd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pjd OCA], [https://pdbe.org/1pjd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pjd RCSB], [https://www.ebi.ac.uk/pdbsum/1pjd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pjd ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pjd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pjd OCA], [https://pdbe.org/1pjd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pjd RCSB], [https://www.ebi.ac.uk/pdbsum/1pjd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pjd ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/STE2_YEAST STE2_YEAST]] Receptor for the peptide pheromone alpha factor, the mating factor of yeast.
+[https://www.uniprot.org/uniprot/STE2_YEAST STE2_YEAST] Receptor for the peptide pheromone alpha factor, the mating factor of yeast.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Arshava, B]]
+[[Category: Saccharomyces cerevisiae]]
-[[Category: Becker, J M]]
+[[Category: Arshava B]]
-[[Category: Ding, F X]]
+[[Category: Becker JM]]
-[[Category: Liu, S F]]
+[[Category: Ding F-X]]
-[[Category: Marassi, F M]]
+[[Category: Liu S-F]]
-[[Category: Naider, F]]
+[[Category: Marassi FM]]
-[[Category: Nevzorov, A A]]
+[[Category: Naider F]]
-[[Category: Opella, S J]]
+[[Category: Nevzorov AA]]
-[[Category: Valentine, K G]]
+[[Category: Opella SJ]]
-[[Category: Veglia, G]]
+[[Category: Valentine KG]]
-[[Category: Wang, S H]]
+[[Category: Veglia G]]
-[[Category: Alpha helix]]
+[[Category: Wang S-H]]
-[[Category: Membrane protein]]

1pjd

From Proteopedia

Current revision

Structure and Topology of a Peptide Segment of the 6th Transmembrane Domain of the Saccharomyces cerevisiae alpha-Factor Receptor in Phospholipid Bilayers

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox