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5gou

From Proteopedia

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Structure of a 16-mer protein nanocage fabricated from its 24-mer analogue by subunit interface redesign

Structural highlights

5gou is a 16 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.91Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FRIH_HUMAN Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).

Publication Abstract from PubMed

Protein assemblies with high symmetry are widely distributed in nature. Most efforts so far have focused on repurposing these protein assemblies, a strategy that is ultimately limited by the structures available. To overcome this limitation, methods for fabricating novel self-assembling proteins have received intensive interest. Herein, by reengineering the key subunit interfaces of native 24-mer protein cage with octahedral symmetry through amino acid residues insertion, we fabricated a 16-mer lenticular nanocage whose structure is unique among all known protein cages. This newly non-native protein can be used for encapsulation of bioactive compounds and exhibits high uptake efficiency by cancer cells. More importantly, the above strategy could be applied to other naturally occurring protein assemblies with high symmetry, leading to the generation of new proteins with unexplored functions.

Conversion of the Native 24-mer Ferritin Nanocage into Its Non-Native 16-mer Analogue by Insertion of Extra Amino Acid Residues.,Zhang S, Zang J, Wang W, Chen H, Zhang X, Wang F, Wang H, Zhao G Angew Chem Int Ed Engl. 2016 Dec 23;55(52):16064-16070. doi:, 10.1002/anie.201609517. Epub 2016 Nov 25. PMID:27885765^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhang S, Zang J, Wang W, Chen H, Zhang X, Wang F, Wang H, Zhao G. Conversion of the Native 24-mer Ferritin Nanocage into Its Non-Native 16-mer Analogue by Insertion of Extra Amino Acid Residues. Angew Chem Int Ed Engl. 2016 Dec 23;55(52):16064-16070. doi:, 10.1002/anie.201609517. Epub 2016 Nov 25. PMID:27885765 doi:http://dx.doi.org/10.1002/anie.201609517

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5gou"

@@ Line 3: / Line 3: @@
 <StructureSection load='5gou' size='340' side='right'caption='[[5gou]], [[Resolution|resolution]] 2.91&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5gou]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5GOU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5gou]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GOU FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FTH1, FTH, FTHL6, OK/SW-cl.84, PIG15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.91&#8491;</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ferroxidase Ferroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.16.3.1 1.16.3.1] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gou OCA], [https://pdbe.org/5gou PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gou RCSB], [https://www.ebi.ac.uk/pdbsum/5gou PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gou ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5gou FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gou OCA], [http://pdbe.org/5gou PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5gou RCSB], [http://www.ebi.ac.uk/pdbsum/5gou PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5gou ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/FRIH_HUMAN FRIH_HUMAN]] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).
+[https://www.uniprot.org/uniprot/FRIH_HUMAN FRIH_HUMAN] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 26: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Ferroxidase]]
+[[Category: Homo sapiens]]
-[[Category: Human]]
 [[Category: Large Structures]]
-[[Category: Wang, H]]
+[[Category: Wang H]]
-[[Category: Wang, W]]
+[[Category: Wang W]]
-[[Category: Zang, J]]
+[[Category: Zang J]]
-[[Category: Zhang, S]]
+[[Category: Zhang S]]
-[[Category: Zhao, G]]
+[[Category: Zhao G]]
-[[Category: 16-mer nanocage]]
-[[Category: Ferritin]]
-[[Category: Oxidoreductase]]
-[[Category: Subunit interface redesign]]

5gou

From Proteopedia

Current revision

Structure of a 16-mer protein nanocage fabricated from its 24-mer analogue by subunit interface redesign

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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